In vivo experiments with mesothelial cell seeded ePTFE vascular grafts

H.J.M. Verhagen; J.D. Blankensteijn; P.G. Groot, de; G.J. Heijnen-Snyder; A. Pronk; T.M. Vroom; J.J. Muller; K. Nicolaij; J.J. Sixma; B.C. Eikelboom

doi:10.1016/S1078-5884(98)80108-1

In vivo experiments with mesothelial cell seeded ePTFE vascular grafts

H.J.M. Verhagen
, J.D. Blankensteijn
, P.G. Groot, de
, G.J. Heijnen-Snyder
, A. Pronk
, T.M. Vroom
, J.J. Muller
, K. Nicolaij
, J.J. Sixma
, B.C. Eikelboom

Mechanical Engineering

Research output: Contribution to journal › Article › Academic › peer-review

22 Citations (Scopus)

Abstract

Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima formation (0.22–1.34 mm) than the control grafts (

Original language	English
Pages (from-to)	489-496
Journal	European Journal of Vascular and Endovascular Surgery
Volume	15
Issue number	6
DOIs	https://doi.org/10.1016/S1078-5884(98)80108-1
Publication status	Published - 1998

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@article{83114a9cfae34365b673a5cfa5ce8d38,

title = "In vivo experiments with mesothelial cell seeded ePTFE vascular grafts",

abstract = "Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima formation (0.22{\textendash}1.34 mm) than the control grafts (",

author = "H.J.M. Verhagen and J.D. Blankensteijn and \{Groot, de\}, P.G. and G.J. Heijnen-Snyder and A. Pronk and T.M. Vroom and J.J. Muller and K. Nicolaij and J.J. Sixma and B.C. Eikelboom",

year = "1998",

doi = "10.1016/S1078-5884(98)80108-1",

language = "English",

volume = "15",

pages = "489--496",

journal = "European Journal of Vascular and Endovascular Surgery",

issn = "1078-5884",

publisher = "W.B. Saunders Ltd",

number = "6",

}

TY - JOUR

T1 - In vivo experiments with mesothelial cell seeded ePTFE vascular grafts

AU - Verhagen, H.J.M.

AU - Blankensteijn, J.D.

AU - Groot, de, P.G.

AU - Heijnen-Snyder, G.J.

AU - Pronk, A.

AU - Vroom, T.M.

AU - Muller, J.J.

AU - Nicolaij, K.

AU - Sixma, J.J.

AU - Eikelboom, B.C.

PY - 1998

Y1 - 1998

N2 - Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima formation (0.22–1.34 mm) than the control grafts (

AB - Objectives: To investigate the influence of mesothelial cell (MC) seeding on patency and neointimal formation of small diameter ePTFE grafts in a canine model. Materials and Methods: MC were isolated from the omentum, cultured, seeded on fibronectin-coated ePTFE grafts (4 cm, 4 mm ID), and implanted in the carotid artery of five Beagle dogs. Each dog also received a non-seeded control graft. Patency was assessed by palpation immediately after implantation, and non-invasively by magnetic resonance angiography (MRA) after 1 week and just prior to sacrifice (4 weeks). Intimal thickness was quantified on histological sections by use of computer-aided morphometry. Results: All grafts were patent after implantation. After 1 week, MRA showed the loss of lumen diameter in two seeded grafts. After 4 weeks, two seeded grafts were occluded, one seeded graft was severely stenosed, and all others were without angiographic lumen reduction. Histology and morphometry confirmed that two seeded grafts were occluded, and demonstrated that the other three seeded grafts showed significantly more intima formation (0.22–1.34 mm) than the control grafts (

U2 - 10.1016/S1078-5884(98)80108-1

DO - 10.1016/S1078-5884(98)80108-1

M3 - Article

SN - 1078-5884

VL - 15

SP - 489

EP - 496

JO - European Journal of Vascular and Endovascular Surgery

JF - European Journal of Vascular and Endovascular Surgery

IS - 6

ER -

In vivo experiments with mesothelial cell seeded ePTFE vascular grafts

Abstract

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