In vivo and in vitro approaches reveal novel insight into the ability of epicardium-derived cells to create their own extracellular environment

Noortje A.M. Bax, Sjoerd N. Duim, Boudewijn P.T. Kruithof, Anke M Smits, Carlijn V.C. Bouten, Marie José Goumans (Corresponding author)

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Abstract

Human epicardium-derived cells (hEPDCs) transplanted in the NOD-SCID mouse heart after myocardial infarction (MI) are known to improve cardiac function, most likely orchestrated by paracrine mechanisms that limit adverse remodeling. It is not yet known, however, if hEPDCs contribute to preservation of cardiac function via the secretion of matrix proteins and/or matrix proteases to reduce scar formation. This study describes the ability of hEPDCs to produce human collagen type I after transplantation into the infarct border zone, thereby creating their own extracellular environment. As the in vivo environment is too complex to investigate the mechanisms involved, we use an in vitro set-up, mimicking biophysical and biochemical cues from the myocardial tissue to unravel hEPDC-induced matrix remodeling. The in vivo contribution of hEPDCs to the cardiac extracellular matrix (ECM) was assessed in a historical dataset of the NOD-SCID murine model of experimentally induced MI and cell transplantation. Analysis showed that within 48 h after transplantation, hEPDCs produce human collagen type I. The build-up of the human collagen microenvironment was reversed within 6 weeks. To understand the hEPDCs response to the pathologic cardiac microenvironment, we studied the influence of cyclic straining and/or transforming growth beta (TGFβ) signaling in vitro. We revealed that 48 h of cyclic straining induced collagen type I production via the TGFβ/ALK5 signaling pathway. The in vitro approach enables further unraveling of the hEPDCs ability to secrete matrix proteins and matrix proteases and the potential to create and remodel the cardiac matrix in response to injury.

Original languageEnglish
Article number81
Number of pages14
JournalFrontiers in Cardiovascular Medicine
Volume6
DOIs
Publication statusPublished - 19 Jun 2019

Fingerprint

Aptitude
Pericardium
Collagen Type I
In Vitro Techniques
Peptide Hydrolases
Transplantation
Myocardial Infarction
Inbred NOD Mouse
SCID Mice
Cell Transplantation
Cicatrix
Cues
Extracellular Matrix
Proteins
Collagen

Keywords

  • epicardium-derived cells
  • extracellular matrix (ECM)
  • mechanosensitivity
  • cardiac fibrosis
  • cardiac remodeling
  • cardiac repair

Cite this

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title = "In vivo and in vitro approaches reveal novel insight into the ability of epicardium-derived cells to create their own extracellular environment",
abstract = "Human epicardium-derived cells (hEPDCs) transplanted in the NOD-SCID mouse heart after myocardial infarction (MI) are known to improve cardiac function, most likely orchestrated by paracrine mechanisms that limit adverse remodeling. It is not yet known, however, if hEPDCs contribute to preservation of cardiac function via the secretion of matrix proteins and/or matrix proteases to reduce scar formation. This study describes the ability of hEPDCs to produce human collagen type I after transplantation into the infarct border zone, thereby creating their own extracellular environment. As the in vivo environment is too complex to investigate the mechanisms involved, we use an in vitro set-up, mimicking biophysical and biochemical cues from the myocardial tissue to unravel hEPDC-induced matrix remodeling. The in vivo contribution of hEPDCs to the cardiac extracellular matrix (ECM) was assessed in a historical dataset of the NOD-SCID murine model of experimentally induced MI and cell transplantation. Analysis showed that within 48 h after transplantation, hEPDCs produce human collagen type I. The build-up of the human collagen microenvironment was reversed within 6 weeks. To understand the hEPDCs response to the pathologic cardiac microenvironment, we studied the influence of cyclic straining and/or transforming growth beta (TGFβ) signaling in vitro. We revealed that 48 h of cyclic straining induced collagen type I production via the TGFβ/ALK5 signaling pathway. The in vitro approach enables further unraveling of the hEPDCs ability to secrete matrix proteins and matrix proteases and the potential to create and remodel the cardiac matrix in response to injury.",
keywords = "epicardium-derived cells, extracellular matrix (ECM), mechanosensitivity, cardiac fibrosis, cardiac remodeling, cardiac repair",
author = "Bax, {Noortje A.M.} and Duim, {Sjoerd N.} and Kruithof, {Boudewijn P.T.} and Smits, {Anke M} and Bouten, {Carlijn V.C.} and Goumans, {Marie Jos{\'e}}",
year = "2019",
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doi = "10.3389/fcvm.2019.00081",
language = "English",
volume = "6",
journal = "Frontiers in Cardiovascular Medicine",
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}

In vivo and in vitro approaches reveal novel insight into the ability of epicardium-derived cells to create their own extracellular environment. / Bax, Noortje A.M.; Duim, Sjoerd N.; Kruithof, Boudewijn P.T.; Smits, Anke M; Bouten, Carlijn V.C.; Goumans, Marie José (Corresponding author).

In: Frontiers in Cardiovascular Medicine, Vol. 6, 81, 19.06.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Bax, Noortje A.M.

AU - Duim, Sjoerd N.

AU - Kruithof, Boudewijn P.T.

AU - Smits, Anke M

AU - Bouten, Carlijn V.C.

AU - Goumans, Marie José

PY - 2019/6/19

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N2 - Human epicardium-derived cells (hEPDCs) transplanted in the NOD-SCID mouse heart after myocardial infarction (MI) are known to improve cardiac function, most likely orchestrated by paracrine mechanisms that limit adverse remodeling. It is not yet known, however, if hEPDCs contribute to preservation of cardiac function via the secretion of matrix proteins and/or matrix proteases to reduce scar formation. This study describes the ability of hEPDCs to produce human collagen type I after transplantation into the infarct border zone, thereby creating their own extracellular environment. As the in vivo environment is too complex to investigate the mechanisms involved, we use an in vitro set-up, mimicking biophysical and biochemical cues from the myocardial tissue to unravel hEPDC-induced matrix remodeling. The in vivo contribution of hEPDCs to the cardiac extracellular matrix (ECM) was assessed in a historical dataset of the NOD-SCID murine model of experimentally induced MI and cell transplantation. Analysis showed that within 48 h after transplantation, hEPDCs produce human collagen type I. The build-up of the human collagen microenvironment was reversed within 6 weeks. To understand the hEPDCs response to the pathologic cardiac microenvironment, we studied the influence of cyclic straining and/or transforming growth beta (TGFβ) signaling in vitro. We revealed that 48 h of cyclic straining induced collagen type I production via the TGFβ/ALK5 signaling pathway. The in vitro approach enables further unraveling of the hEPDCs ability to secrete matrix proteins and matrix proteases and the potential to create and remodel the cardiac matrix in response to injury.

AB - Human epicardium-derived cells (hEPDCs) transplanted in the NOD-SCID mouse heart after myocardial infarction (MI) are known to improve cardiac function, most likely orchestrated by paracrine mechanisms that limit adverse remodeling. It is not yet known, however, if hEPDCs contribute to preservation of cardiac function via the secretion of matrix proteins and/or matrix proteases to reduce scar formation. This study describes the ability of hEPDCs to produce human collagen type I after transplantation into the infarct border zone, thereby creating their own extracellular environment. As the in vivo environment is too complex to investigate the mechanisms involved, we use an in vitro set-up, mimicking biophysical and biochemical cues from the myocardial tissue to unravel hEPDC-induced matrix remodeling. The in vivo contribution of hEPDCs to the cardiac extracellular matrix (ECM) was assessed in a historical dataset of the NOD-SCID murine model of experimentally induced MI and cell transplantation. Analysis showed that within 48 h after transplantation, hEPDCs produce human collagen type I. The build-up of the human collagen microenvironment was reversed within 6 weeks. To understand the hEPDCs response to the pathologic cardiac microenvironment, we studied the influence of cyclic straining and/or transforming growth beta (TGFβ) signaling in vitro. We revealed that 48 h of cyclic straining induced collagen type I production via the TGFβ/ALK5 signaling pathway. The in vitro approach enables further unraveling of the hEPDCs ability to secrete matrix proteins and matrix proteases and the potential to create and remodel the cardiac matrix in response to injury.

KW - epicardium-derived cells

KW - extracellular matrix (ECM)

KW - mechanosensitivity

KW - cardiac fibrosis

KW - cardiac remodeling

KW - cardiac repair

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DO - 10.3389/fcvm.2019.00081

M3 - Article

C2 - 31275946

VL - 6

JO - Frontiers in Cardiovascular Medicine

JF - Frontiers in Cardiovascular Medicine

SN - 2297-055X

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