In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates

E. Lamers, X.F. Walboomers, M. Domanski, L. Prodanov, J. Melis, R. Luttge, A.J.A. Winnubst, J.M. Anderson, J.G.E. Gardeniers, J.A. Jansen

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Abstract

The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1ß and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1ß, TNF-a, TGF-ß and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.
LanguageEnglish
Pages308-317
JournalNanomedicine
Volume8
Issue number3
DOIs
StatePublished - 2012

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Macrophages
Interleukins
Biocompatible Materials
Cytokines
Biomaterials
Osteopontin
Macrophage Activation
Substrates
Cell Adhesion
Wound Healing
Extracellular Matrix
Lasers
Cell Culture Techniques
Texturing
Cell adhesion
Reactive ion etching
Ions
Cell culture
Ion implantation
Lithography

Cite this

Lamers, E., Walboomers, X. F., Domanski, M., Prodanov, L., Melis, J., Luttge, R., ... Jansen, J. A. (2012). In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. Nanomedicine, 8(3), 308-317. DOI: 10.1016/j.nano.2011.06.013
Lamers, E. ; Walboomers, X.F. ; Domanski, M. ; Prodanov, L. ; Melis, J. ; Luttge, R. ; Winnubst, A.J.A. ; Anderson, J.M. ; Gardeniers, J.G.E. ; Jansen, J.A./ In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. In: Nanomedicine. 2012 ; Vol. 8, No. 3. pp. 308-317
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Lamers, E, Walboomers, XF, Domanski, M, Prodanov, L, Melis, J, Luttge, R, Winnubst, AJA, Anderson, JM, Gardeniers, JGE & Jansen, JA 2012, 'In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates' Nanomedicine, vol. 8, no. 3, pp. 308-317. DOI: 10.1016/j.nano.2011.06.013

In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. / Lamers, E.; Walboomers, X.F.; Domanski, M.; Prodanov, L.; Melis, J.; Luttge, R.; Winnubst, A.J.A.; Anderson, J.M.; Gardeniers, J.G.E.; Jansen, J.A.

In: Nanomedicine, Vol. 8, No. 3, 2012, p. 308-317.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Walboomers,X.F.

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AB - The immune response to an implanted biomaterial is orchestrated by macrophages. In this study various nanogrooved patterns were created by using laser interference lithography and reactive ion etching. The created nanogrooves mimic the natural extracellular matrix environment. Macrophage cell culture demonstrated that interleukin 1ß and TNF-a cytokine production were upregulated on nanogrooved substrates. In vivo subcutaneous implantation in a validated mouse cage model for 14 days demonstrated that nanogrooves enhanced and guided cell adhesion, and few multinucleated cells were formed. In agreement with the in vitro results, cytokine production was found to be nanogroove dependent, as interleukin 1ß, TNF-a, TGF-ß and osteopontin became upregulated. The results indicate that biomaterial surface texturing, especially at the nanometric scale, can be used to control macrophage activation to induce a wound healing response, rather than a profound inflammatory response.

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Lamers E, Walboomers XF, Domanski M, Prodanov L, Melis J, Luttge R et al. In vitro and in vivo evaluation of the inflammatory response to nanoscale grooved substrates. Nanomedicine. 2012;8(3):308-317. Available from, DOI: 10.1016/j.nano.2011.06.013