In-situ optical detection of mesoscale components in glass microfluidic channel with monolithic waveguide

V.K. Pahilwani, Y.J. Bellouard, A.A. Said, M. Dugan, P. Bado

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

7 Citations (Scopus)


Optical waveguides used as a local light source along a fluidic channel have proven to be an effective approach to detecting cells in the field of flow-cytometry. One challenge, however, has been a simple integration of optical waveguides with the fluidic channel. We employ the use of femtosecond laser-writing process to pattern a waveguide in the bulk of a fused-silica glass substrate housing a fluidic channel. We demonstrate an in-situ scheme for detecting sub-millimeter components based on such a monolithically fabricated device. By illuminating the waveguide and collecting the light signal past the channel, we detect opaque and transparent components between 300 - 500 µm in size, as each moves along the channel. Both an opaque square chip and a transparent bead attentuate the signal by more than 95% primarily due to reflection and refraction respectively. The signature of a transparent bead additionally shows attenuated peaks which we attribute to normal incidence of light from the waveguide. The projected sizes of the parts are determined with less than 1% uncertainty. We conclude that the femtosecond laser produced waveguides in fused-silica glass are a viable option for the detection of certain kinds of sub-millimeter components. This approach holds the prospects of fabricating complex three-dimensional networks of waveguides monolithically.
Original languageEnglish
Title of host publicationOptomechatronic Actuators and Manipulation III, Lausanne, 2007
EditorsY. Bellouard, Y. Otani, K.S. Moon
Place of PublicationBellingham
Publication statusPublished - 2007

Publication series

NameProceedings of SPIE
ISSN (Print)0277-786X


Dive into the research topics of 'In-situ optical detection of mesoscale components in glass microfluidic channel with monolithic waveguide'. Together they form a unique fingerprint.

Cite this