Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

Hanna Talacua; Serge H M Söntjens; Shraddha H Thakkar; Aurelie M A Brizard; Lex A van Herwerden; Aryan Vink; Geert C van Almen; Patricia Y W Dankers; Carlijn V C Bouten; Ricardo P J Budde; Henk M Janssen; Jolanda Kluin

doi:10.1002/mabi.202000024

Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

Hanna Talacua, Serge H M Söntjens, Shraddha H Thakkar, Aurelie M A Brizard, Lex A van Herwerden, Aryan Vink, Geert C van Almen, Patricia Y W Dankers (Corresponding author), Carlijn V C Bouten, Ricardo P J Budde, Henk M Janssen, Jolanda Kluin

Research output: Contribution to journal › Article › Academic › peer-review

11 Citations (Scopus)

Abstract

For in situ tissue engineering (TE) applications it is important that implant degradation proceeds in concord with neo-tissue formation to avoid graft failure. It will therefore be valuable to have an imaging contrast agent (CA) available that can report on the degrading implant. For this purpose, a biodegradable radiopaque biomaterial is presented, modularly composed of a bisurea chain-extended polycaprolactone (PCL2000-U4U) elastomer and a novel iodinated bisurea-modified CA additive (I-U4U). Supramolecular hydrogen bonding interactions between the components ensure their intimate mixing. Porous implant TE-grafts are prepared by simply electrospinning a solution containing PCL2000-U4U and I-U4U. Rats receive an aortic interposition graft, either composed of only PCL2000-U4U (control) or of PCL2000-U4U and I-U4U (test). The grafts are explanted for analysis at three time points over a 1-month period. Computed tomography imaging of the test group implants prior to explantation shows a decrease in iodide volume and density over time. Explant analysis also indicates scaffold degradation. (Immuno)histochemistry shows comparable cellular contents and a similar neo-tissue formation process for test and control group, demonstrating that the CA does not have apparent adverse effects. A supramolecular approach to create solid radiopaque biomaterials can therefore be used to noninvasively monitor the biodegradation of synthetic implants.

Original language	English
Article number	2000024
Number of pages	14
Journal	Macromolecular Bioscience
Volume	20
Issue number	7
Early online date	17 Jun 2020
DOIs	https://doi.org/10.1002/mabi.202000024
Publication status	Published - 1 Jul 2020

Bibliographical note

Funding

This research formed part of the iValve project of the research program of the BioMedical Materials institute, co-founded by the Dutch Ministry of Economic Affairs, Agriculture and Innovation. The financial contribution of the Nederlandse Hartstichting is most gratefully acknowledged. All members of the iValve project are thanked for valuable discussions. Joost L.J. van Dongen, Xianwen Lou (from the Macromolecular and Organic Chemistry group at the TU/e Eindhoven, the Netherlands) and Michel Fransen (SyMO-Chem BV) are thanked for their contributions and help on MS (HRMS, MALDI-TOF-MS, HPLC-MS and GC-MS) and GPC measurements. This research formed part of the iValve project of the research program of the BioMedical Materials institute, co‐founded by the Dutch Ministry of Economic Affairs, Agriculture and Innovation. The financial contribution of the Nederlandse Hartstichting is most gratefully acknowledged. All members of the iValve project are thanked for valuable discussions. Joost L.J. van Dongen, Xianwen Lou (from the Macromolecular and Organic Chemistry group at the TU/e Eindhoven, the Netherlands) and Michel Fransen (SyMO‐Chem BV) are thanked for their contributions and help on MS (HRMS, MALDI‐TOF‐MS, HPLC‐MS and GC‐MS) and GPC measurements.

Funders	Funder number
Ministerie van Economische Zaken en Klimaat

Keywords

aortic interposition graft implants
computed tomography imaging
degrading supramolecular biomaterials
modular contrast agents
tissue engineering

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Talacua, H., Söntjens, S. H. M., Thakkar, S. H., Brizard, A. M. A., van Herwerden, L. A., Vink, A., van Almen, G. C., Dankers, P. Y. W., Bouten, C. V. C., Budde, R. P. J., Janssen, H. M., & Kluin, J. (2020). Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials. Macromolecular Bioscience, 20(7), Article 2000024. https://doi.org/10.1002/mabi.202000024

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abstract = "For in situ tissue engineering (TE) applications it is important that implant degradation proceeds in concord with neo-tissue formation to avoid graft failure. It will therefore be valuable to have an imaging contrast agent (CA) available that can report on the degrading implant. For this purpose, a biodegradable radiopaque biomaterial is presented, modularly composed of a bisurea chain-extended polycaprolactone (PCL2000-U4U) elastomer and a novel iodinated bisurea-modified CA additive (I-U4U). Supramolecular hydrogen bonding interactions between the components ensure their intimate mixing. Porous implant TE-grafts are prepared by simply electrospinning a solution containing PCL2000-U4U and I-U4U. Rats receive an aortic interposition graft, either composed of only PCL2000-U4U (control) or of PCL2000-U4U and I-U4U (test). The grafts are explanted for analysis at three time points over a 1-month period. Computed tomography imaging of the test group implants prior to explantation shows a decrease in iodide volume and density over time. Explant analysis also indicates scaffold degradation. (Immuno)histochemistry shows comparable cellular contents and a similar neo-tissue formation process for test and control group, demonstrating that the CA does not have apparent adverse effects. A supramolecular approach to create solid radiopaque biomaterials can therefore be used to noninvasively monitor the biodegradation of synthetic implants.",

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Talacua, H, Söntjens, SHM, Thakkar, SH, Brizard, AMA, van Herwerden, LA, Vink, A, van Almen, GC, Dankers, PYW , Bouten, CVC, Budde, RPJ, Janssen, HM & Kluin, J 2020, 'Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials', Macromolecular Bioscience, vol. 20, no. 7, 2000024. https://doi.org/10.1002/mabi.202000024

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AU - Söntjens, Serge H M

AU - Thakkar, Shraddha H

AU - Brizard, Aurelie M A

AU - van Herwerden, Lex A

AU - Vink, Aryan

AU - van Almen, Geert C

AU - Dankers, Patricia Y W

AU - Bouten, Carlijn V C

AU - Budde, Ricardo P J

AU - Janssen, Henk M

AU - Kluin, Jolanda

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Imaging the In Vivo Degradation of Tissue Engineering Implants by Use of Supramolecular Radiopaque Biomaterials

Abstract

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Keywords

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