Identification of Malaysia's Edible Bird's Nest geographical origin using gel electrophoresis analysis

Ting Hun Lee; Syieluing Wong; Chia Hau Lee; Nurul Alia Azmi; Murugiah Darshini; Supparmaniam Kavita; Kian Kai Cheng

doi:10.12982/CMUJNS.2020.0025

Identification of Malaysia's Edible Bird's Nest geographical origin using gel electrophoresis analysis

Ting Hun Lee (Corresponding author), Syieluing Wong, Chia Hau Lee, Nurul Alia Azmi, Murugiah Darshini, Supparmaniam Kavita, Kian Kai Cheng

Research output: Contribution to journal › Article › Academic › peer-review

8 Citations (Scopus)

Abstract

There is an urgent need to develop a rapid and robust approach to ensure traceability of the Edible Bird's Nest (EBN) products. The main composition of EBN is protein and it plays an important role in EBN. This study shows that there are significant differences in the protein profile of EBN collected from different states in Malaysia. The SDS-PAGE method is used to analyse the protein profile for 65 samples collected from 13 different states of Malaysia. SDS-PAGE gel image showed that all EBN samples analyzed have been well separated between protein bands. Data shows that protein band at 212 kD can only be detected in state P, while 135 kD can only be found in state M. EBN from other states have their unique combination of fingerprints that made them different from one to another. This research demonstrated the possibility of using SDS-PAGE pattern for identification and differentiation of the geographical origins of EBN produced in Malaysia. These findings are the first of its kind in EBN analysis.

Original language	English
Pages (from-to)	379-392
Number of pages	14
Journal	Chiang Mai University Journal of Natural Sciences
Volume	19
Issue number	3
DOIs	https://doi.org/10.12982/CMUJNS.2020.0025
Publication status	Published - 1 Jul 2020
Externally published	Yes

Bibliographical note

Funding Information:
This study was supported by the Research University grant (GUP, grant number 10J74).

Funding

This study was supported by the Research University grant (GUP, grant number 10J74).

Keywords

Edible bird's nest
Geographical origin
Identification
Protein profiling
Sodium Dodecyl Sulfate Polyacrylamide Gel electrophoresis (SDS-PAGE)

Access to Document

10.12982/CMUJNS.2020.0025

Cite this

@article{8f7fb69450534402b0e862a27a2c59a7,

title = "Identification of Malaysia's Edible Bird's Nest geographical origin using gel electrophoresis analysis",

abstract = "There is an urgent need to develop a rapid and robust approach to ensure traceability of the Edible Bird's Nest (EBN) products. The main composition of EBN is protein and it plays an important role in EBN. This study shows that there are significant differences in the protein profile of EBN collected from different states in Malaysia. The SDS-PAGE method is used to analyse the protein profile for 65 samples collected from 13 different states of Malaysia. SDS-PAGE gel image showed that all EBN samples analyzed have been well separated between protein bands. Data shows that protein band at 212 kD can only be detected in state P, while 135 kD can only be found in state M. EBN from other states have their unique combination of fingerprints that made them different from one to another. This research demonstrated the possibility of using SDS-PAGE pattern for identification and differentiation of the geographical origins of EBN produced in Malaysia. These findings are the first of its kind in EBN analysis.",

keywords = "Edible bird's nest, Geographical origin, Identification, Protein profiling, Sodium Dodecyl Sulfate Polyacrylamide Gel electrophoresis (SDS-PAGE)",

author = "Lee, {Ting Hun} and Syieluing Wong and Lee, {Chia Hau} and Azmi, {Nurul Alia} and Murugiah Darshini and Supparmaniam Kavita and Cheng, {Kian Kai}",

note = "Funding Information: This study was supported by the Research University grant (GUP, grant number 10J74). ",

year = "2020",

month = jul,

day = "1",

doi = "10.12982/CMUJNS.2020.0025",

language = "English",

volume = "19",

pages = "379--392",

journal = "Chiang Mai University Journal of Natural Sciences",

issn = "1685-1994",

publisher = "Chiang Mai University",

number = "3",

}

TY - JOUR

T1 - Identification of Malaysia's Edible Bird's Nest geographical origin using gel electrophoresis analysis

AU - Lee, Ting Hun

AU - Wong, Syieluing

AU - Lee, Chia Hau

AU - Azmi, Nurul Alia

AU - Darshini, Murugiah

AU - Kavita, Supparmaniam

AU - Cheng, Kian Kai

N1 - Funding Information: This study was supported by the Research University grant (GUP, grant number 10J74).

PY - 2020/7/1

Y1 - 2020/7/1

N2 - There is an urgent need to develop a rapid and robust approach to ensure traceability of the Edible Bird's Nest (EBN) products. The main composition of EBN is protein and it plays an important role in EBN. This study shows that there are significant differences in the protein profile of EBN collected from different states in Malaysia. The SDS-PAGE method is used to analyse the protein profile for 65 samples collected from 13 different states of Malaysia. SDS-PAGE gel image showed that all EBN samples analyzed have been well separated between protein bands. Data shows that protein band at 212 kD can only be detected in state P, while 135 kD can only be found in state M. EBN from other states have their unique combination of fingerprints that made them different from one to another. This research demonstrated the possibility of using SDS-PAGE pattern for identification and differentiation of the geographical origins of EBN produced in Malaysia. These findings are the first of its kind in EBN analysis.

AB - There is an urgent need to develop a rapid and robust approach to ensure traceability of the Edible Bird's Nest (EBN) products. The main composition of EBN is protein and it plays an important role in EBN. This study shows that there are significant differences in the protein profile of EBN collected from different states in Malaysia. The SDS-PAGE method is used to analyse the protein profile for 65 samples collected from 13 different states of Malaysia. SDS-PAGE gel image showed that all EBN samples analyzed have been well separated between protein bands. Data shows that protein band at 212 kD can only be detected in state P, while 135 kD can only be found in state M. EBN from other states have their unique combination of fingerprints that made them different from one to another. This research demonstrated the possibility of using SDS-PAGE pattern for identification and differentiation of the geographical origins of EBN produced in Malaysia. These findings are the first of its kind in EBN analysis.

KW - Edible bird's nest

KW - Geographical origin

KW - Identification

KW - Protein profiling

KW - Sodium Dodecyl Sulfate Polyacrylamide Gel electrophoresis (SDS-PAGE)

UR - http://www.scopus.com/inward/record.url?scp=85089013455&partnerID=8YFLogxK

U2 - 10.12982/CMUJNS.2020.0025

DO - 10.12982/CMUJNS.2020.0025

M3 - Article

AN - SCOPUS:85089013455

SN - 1685-1994

VL - 19

SP - 379

EP - 392

JO - Chiang Mai University Journal of Natural Sciences

JF - Chiang Mai University Journal of Natural Sciences

IS - 3

ER -

Identification of Malaysia's Edible Bird's Nest geographical origin using gel electrophoresis analysis

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this