Highly efficient enzyme encapsulation in a protein nanocage: towards enzyme catalysis in a cellular nanocompartment mimic

L. Schoonen, R.J.M. Nolte, J.C.M. van Hest

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)
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Abstract

The study of enzyme behavior in small nanocompartments is crucial for the understanding of biocatalytic processes in the cellular environment. We have developed an enzymatic conjugation strategy to attach a model enzyme to the interior of a cowpea chlorotic mottle virus capsid. It is shown that with this methodology high encapsulation efficiencies can be achieved. Additionally, we demonstrate that the encapsulation does not affect the enzyme performance in terms of a decreased activity or a hampered substrate diffusion. Finally, it is shown that the encapsulated enzymes are protected against proteases. We believe that our strategy can be used to study enzyme kinetics in an environment that approaches physiological conditions.
Original languageEnglish
Article number6
Pages (from-to)14467-14472
JournalNanoscale
Volume8
Issue number30
DOIs
Publication statusPublished - 6 Jul 2016
Externally publishedYes

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