Hierarchical self-assembly of a copolymer-stabilized coacervate protocell

A.F. Mason, B.C. Buddingh, D.S. Williams, J.C.M. van Hest

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)
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Abstract

Complex coacervate microdroplets are finding increased utility in synthetic cell applications due to their c-ytomimetic properties. However, their intrinsic membrane-free nature results in instability that limits their application in protocell research. Herein, we present the development of a new protocell model through the spontaneous interfacial self-assembly of copolymer molecules on biopolymer coacervate microdroplets. This hierarchical protocell model not only incorporates the favorable properties of coacervates (such as spontaneous assembly and macromolecular condensation) but also assimilates the essential features of a semipermeable copolymeric membrane (such as discretization and stabilization). This was accomplished by engineering an asymmetric, biodegradable triblock copolymer molecule comprising hydrophilic, hydrophobic, and polyanionic components capable of direct coacervate membranization via electrostatic surface anchoring and chain self association. The resulting hierarchical protocell demonstrated striking integrity as a result of membrane formation, successfully stabilizing enzymatic cargo against coalescence and fusion in discrete protocellular populations. The semipermeable nature of the copolymeric membrane enabled the incorporation of a simple enzymatic cascade, demonstrating chemical communication between discrete populations of neighboring protocells. In this way, we pave the way for the development of new synthetic cell constructs.
Original languageEnglish
Article number48
Pages (from-to)17309-17312
Number of pages4
JournalJournal of the American Chemical Society
Volume139
Issue number48
DOIs
Publication statusPublished - 6 Dec 2017

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Artificial Cells
Self assembly
Copolymers
Membranes
Biopolymers
Molecules
Coalescence
Block copolymers
Condensation
Electrostatics
Fusion reactions
Stabilization
Association reactions
Static Electricity
Communication
Population
Research

Cite this

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title = "Hierarchical self-assembly of a copolymer-stabilized coacervate protocell",
abstract = "Complex coacervate microdroplets are finding increased utility in synthetic cell applications due to their c-ytomimetic properties. However, their intrinsic membrane-free nature results in instability that limits their application in protocell research. Herein, we present the development of a new protocell model through the spontaneous interfacial self-assembly of copolymer molecules on biopolymer coacervate microdroplets. This hierarchical protocell model not only incorporates the favorable properties of coacervates (such as spontaneous assembly and macromolecular condensation) but also assimilates the essential features of a semipermeable copolymeric membrane (such as discretization and stabilization). This was accomplished by engineering an asymmetric, biodegradable triblock copolymer molecule comprising hydrophilic, hydrophobic, and polyanionic components capable of direct coacervate membranization via electrostatic surface anchoring and chain self association. The resulting hierarchical protocell demonstrated striking integrity as a result of membrane formation, successfully stabilizing enzymatic cargo against coalescence and fusion in discrete protocellular populations. The semipermeable nature of the copolymeric membrane enabled the incorporation of a simple enzymatic cascade, demonstrating chemical communication between discrete populations of neighboring protocells. In this way, we pave the way for the development of new synthetic cell constructs.",
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Hierarchical self-assembly of a copolymer-stabilized coacervate protocell. / Mason, A.F.; Buddingh, B.C.; Williams, D.S.; van Hest, J.C.M.

In: Journal of the American Chemical Society, Vol. 139, No. 48, 48, 06.12.2017, p. 17309-17312.

Research output: Contribution to journalArticleAcademicpeer-review

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