Graphene liquid cells assembled through loop‐assisted transfer method and located with correlated light‐electron microscopy

Pauline M.G. van Deursen, Roman I. Koning, Viorica Tudor, Mohammad Amin Moradi, Joseph P. Patterson, Alexander Kros, Nico A.J.M. Sommerdijk, Abraham J. Koster, Grégory F. Schneider (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

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Graphene liquid cells (GLCs) for transmission electron microscopy (TEM) enable high‐resolution, real‐time imaging of dynamic processes in water. Large‐scale implementation, however, is prevented by major difficulties in reproducing GLC fabrication. Here, a high‐yield method is presented to fabricate GLCs under millimeter areas of continuous graphene, facilitating efficient GLC formation on a TEM grid. Additionally, GLCs are located on the grid using correlated light‐electron microscopy (CLEM), which reduces beam damage by limiting electron exposure time. CLEM allows the acquisition of reliable statistics and the investigation of the most common shapes of GLCs. In particular, a novel type of liquid cell is found, formed from only a single graphene sheet, greatly simplifying the fabrication process. The methods presented in this work—particularly the reproducibility and simplicity of fabrication—will enable future application of GLCs for high‐resolution dynamic imaging of biomolecular systems.
Original languageEnglish
Article number1904468
Number of pages9
JournalAdvanced Functional Materials
Issue number11
Early online date3 Feb 2020
Publication statusPublished - 1 Mar 2020

Bibliographical note

This article also appears in: Hot Topic: Carbon, Graphite, and Graphene


P.M.G.D. and G.F.S. acknowledge the funding received for this work through the European Research Commission (ERC) Starting Grant and the VIDI Innovational Research Incentives Scheme from the Netherlands Organisation for Scientific Research (NWO). A.J.K. was partly supported by the NWO domain Applied and Engineering Sciences (Stichting Technologische Wetenschappen, STW) Perspectief Grant Microscopy Valley, STW12713. A.J.K. and R.I.K. were partly supported by iNEXT, project number 653706, and by the NWO‐STW project number STW13711. J.P.P. was supported by the Marie Sklodowska‐Curie Action project “LPEMM.” M.‐A.M was supported by the 4TU.High‐Tech Materials research program “New Horizons in designer materials.”

FundersFunder number
European Research Commission
NWO domain Applied and Engineering Sciences
Stichting Technologische Wetenschappen653706, STW12713
Marie Skłodowska‐Curie
Nederlandse Organisatie voor Wetenschappelijk Onderzoek


    • correlated light-electron microscopy
    • graphene liquid cells
    • graphene transfer
    • liquid phase electron microscopy
    • time-resolved electron microscopy


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