Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction

Willem E.M. Noteborn, Joeri A.J. Wondergem, Anastasiia Iurchenko, Farhad Chariyev-Prinz, Dominique Donato, Ilja K. Voets, Doris Heinrich, Roxanne E. Kieltyka

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Abstract

Nucleic acid-polymer conjugates are an attractive class of materials endowed with tunable and responsive character. Herein, we exploit the dynamic character of nucleic acids in the preparation of hybrid DNA-covalent polymers with extendable grafts by the hybridization chain reaction. Addition of DNA hairpins to an initiator DNA-dextran graft copolymer resulted in the growth of the DNA grafts as evidenced by various characterization techniques over several length scales. Additionally, aggregation of the initiator DNA-graft copolymer before the hybridization chain reaction was observed resulting in the formation of kinetically trapped aggregates several hundreds of nanometers in diameter that could be disrupted by a preheating step at 60 °C prior to extension at room temperature. Materials of increasing viscosity were rapidly formed when metastable DNA hairpins were added to the initiator DNA-dextran grafted copolymer with increasing concentration of the components in the mixture. This study shows the potential for hierarchical self-assembly of DNA-grafted polymers through the hybridization chain reaction and opens the door for biomedical applications where viscosity can be used as a readout.

Original languageEnglish
Pages (from-to)5157-5164
Number of pages8
JournalMacromolecules
Volume51
Issue number14
DOIs
Publication statusPublished - 24 Jul 2018

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Polymers
DNA
Dextran
Graft copolymers
Nucleic acids
Dextrans
Grafts
Nucleic Acids
Viscosity
Preheating
Self assembly
Agglomeration
Copolymers

Cite this

Noteborn, W. E. M., Wondergem, J. A. J., Iurchenko, A., Chariyev-Prinz, F., Donato, D., Voets, I. K., ... Kieltyka, R. E. (2018). Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction. Macromolecules, 51(14), 5157-5164. https://doi.org/10.1021/acs.macromol.7b02610
Noteborn, Willem E.M. ; Wondergem, Joeri A.J. ; Iurchenko, Anastasiia ; Chariyev-Prinz, Farhad ; Donato, Dominique ; Voets, Ilja K. ; Heinrich, Doris ; Kieltyka, Roxanne E. / Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction. In: Macromolecules. 2018 ; Vol. 51, No. 14. pp. 5157-5164.
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Noteborn, WEM, Wondergem, JAJ, Iurchenko, A, Chariyev-Prinz, F, Donato, D, Voets, IK, Heinrich, D & Kieltyka, RE 2018, 'Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction', Macromolecules, vol. 51, no. 14, pp. 5157-5164. https://doi.org/10.1021/acs.macromol.7b02610

Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction. / Noteborn, Willem E.M.; Wondergem, Joeri A.J.; Iurchenko, Anastasiia; Chariyev-Prinz, Farhad; Donato, Dominique; Voets, Ilja K.; Heinrich, Doris; Kieltyka, Roxanne E.

In: Macromolecules, Vol. 51, No. 14, 24.07.2018, p. 5157-5164.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Chariyev-Prinz, Farhad

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AB - Nucleic acid-polymer conjugates are an attractive class of materials endowed with tunable and responsive character. Herein, we exploit the dynamic character of nucleic acids in the preparation of hybrid DNA-covalent polymers with extendable grafts by the hybridization chain reaction. Addition of DNA hairpins to an initiator DNA-dextran graft copolymer resulted in the growth of the DNA grafts as evidenced by various characterization techniques over several length scales. Additionally, aggregation of the initiator DNA-graft copolymer before the hybridization chain reaction was observed resulting in the formation of kinetically trapped aggregates several hundreds of nanometers in diameter that could be disrupted by a preheating step at 60 °C prior to extension at room temperature. Materials of increasing viscosity were rapidly formed when metastable DNA hairpins were added to the initiator DNA-dextran grafted copolymer with increasing concentration of the components in the mixture. This study shows the potential for hierarchical self-assembly of DNA-grafted polymers through the hybridization chain reaction and opens the door for biomedical applications where viscosity can be used as a readout.

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Noteborn WEM, Wondergem JAJ, Iurchenko A, Chariyev-Prinz F, Donato D, Voets IK et al. Grafting from a Hybrid DNA-covalent polymer by the hybridization chain reaction. Macromolecules. 2018 Jul 24;51(14):5157-5164. https://doi.org/10.1021/acs.macromol.7b02610