Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation

B.W. Lagerveld; P. Horssen, van; M.P. Laguna; J.P.H.M. Wijngaard, van den; M. Siebes; H. Wijkstra; J.J.M.C.H. Rosette, de la; J.A.E. Spaan

doi:10.1016/j.juro.2011.03.146

Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation

B.W. Lagerveld, P. Horssen, van, M.P. Laguna, J.P.H.M. Wijngaard, van den, M. Siebes, H. Wijkstra, J.J.M.C.H. Rosette, de la, J.A.E. Spaan

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Abstract

Purpose We quantified temporal changes in vascular structure and blood flow after cryosurgery of the porcine kidney in vivo. Materials and Methods We studied 5 groups of 4 kidneys each with a survival time of 20 minutes, 4 hours, 2 days, and 1 and 2 weeks after cryoablation, respectively. Before harvesting the kidneys, fluorescently labeled microspheres were administrated in the descending aorta. After harvest the kidney and its vasculature were casted with fluorescently dyed elastomer, frozen and processed in an imaging cryomicrotome to reveal the 3-dimensional arterial branching structure and microsphere distribution. In regions of interest vessels were segmented by image analysis software and histograms were constructed to reveal the total summed vessel length as a function of diameter. A characteristic diameter of the ablated area was measured. Results The 20-minute survival group histograms showed a significant shift of the peak to larger diameters (p

Original language	English
Pages (from-to)	681-686
Journal	Journal of Urology
Volume	186
Issue number	2
DOIs	https://doi.org/10.1016/j.juro.2011.03.146
Publication status	Published - 2011

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title = "Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation",

abstract = "Purpose We quantified temporal changes in vascular structure and blood flow after cryosurgery of the porcine kidney in vivo. Materials and Methods We studied 5 groups of 4 kidneys each with a survival time of 20 minutes, 4 hours, 2 days, and 1 and 2 weeks after cryoablation, respectively. Before harvesting the kidneys, fluorescently labeled microspheres were administrated in the descending aorta. After harvest the kidney and its vasculature were casted with fluorescently dyed elastomer, frozen and processed in an imaging cryomicrotome to reveal the 3-dimensional arterial branching structure and microsphere distribution. In regions of interest vessels were segmented by image analysis software and histograms were constructed to reveal the total summed vessel length as a function of diameter. A characteristic diameter of the ablated area was measured. Results The 20-minute survival group histograms showed a significant shift of the peak to larger diameters (p",

author = "B.W. Lagerveld and {Horssen, van}, P. and M.P. Laguna and {Wijngaard, van den}, J.P.H.M. and M. Siebes and H. Wijkstra and {Rosette, de la}, J.J.M.C.H. and J.A.E. Spaan",

year = "2011",

doi = "10.1016/j.juro.2011.03.146",

language = "English",

volume = "186",

pages = "681--686",

journal = "Journal of Urology",

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TY - JOUR

T1 - Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation

AU - Lagerveld, B.W.

AU - Horssen, van, P.

AU - Laguna, M.P.

AU - Wijngaard, van den, J.P.H.M.

AU - Siebes, M.

AU - Wijkstra, H.

AU - Rosette, de la, J.J.M.C.H.

AU - Spaan, J.A.E.

PY - 2011

Y1 - 2011

N2 - Purpose We quantified temporal changes in vascular structure and blood flow after cryosurgery of the porcine kidney in vivo. Materials and Methods We studied 5 groups of 4 kidneys each with a survival time of 20 minutes, 4 hours, 2 days, and 1 and 2 weeks after cryoablation, respectively. Before harvesting the kidneys, fluorescently labeled microspheres were administrated in the descending aorta. After harvest the kidney and its vasculature were casted with fluorescently dyed elastomer, frozen and processed in an imaging cryomicrotome to reveal the 3-dimensional arterial branching structure and microsphere distribution. In regions of interest vessels were segmented by image analysis software and histograms were constructed to reveal the total summed vessel length as a function of diameter. A characteristic diameter of the ablated area was measured. Results The 20-minute survival group histograms showed a significant shift of the peak to larger diameters (p

AB - Purpose We quantified temporal changes in vascular structure and blood flow after cryosurgery of the porcine kidney in vivo. Materials and Methods We studied 5 groups of 4 kidneys each with a survival time of 20 minutes, 4 hours, 2 days, and 1 and 2 weeks after cryoablation, respectively. Before harvesting the kidneys, fluorescently labeled microspheres were administrated in the descending aorta. After harvest the kidney and its vasculature were casted with fluorescently dyed elastomer, frozen and processed in an imaging cryomicrotome to reveal the 3-dimensional arterial branching structure and microsphere distribution. In regions of interest vessels were segmented by image analysis software and histograms were constructed to reveal the total summed vessel length as a function of diameter. A characteristic diameter of the ablated area was measured. Results The 20-minute survival group histograms showed a significant shift of the peak to larger diameters (p

U2 - 10.1016/j.juro.2011.03.146

DO - 10.1016/j.juro.2011.03.146

M3 - Article

C2 - 21683407

VL - 186

SP - 681

EP - 686

JO - Journal of Urology

JF - Journal of Urology

SN - 0022-5347

IS - 2

ER -

Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation

Abstract

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