Gradient changes in porcine renal arterial vascular anatomy and blood flow after cryoablation

B.W. Lagerveld, P. Horssen, van, M.P. Laguna, J.P.H.M. Wijngaard, van den, M. Siebes, H. Wijkstra, J.J.M.C.H. Rosette, de la, J.A.E. Spaan

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Purpose We quantified temporal changes in vascular structure and blood flow after cryosurgery of the porcine kidney in vivo. Materials and Methods We studied 5 groups of 4 kidneys each with a survival time of 20 minutes, 4 hours, 2 days, and 1 and 2 weeks after cryoablation, respectively. Before harvesting the kidneys, fluorescently labeled microspheres were administrated in the descending aorta. After harvest the kidney and its vasculature were casted with fluorescently dyed elastomer, frozen and processed in an imaging cryomicrotome to reveal the 3-dimensional arterial branching structure and microsphere distribution. In regions of interest vessels were segmented by image analysis software and histograms were constructed to reveal the total summed vessel length as a function of diameter. A characteristic diameter of the ablated area was measured. Results The 20-minute survival group histograms showed a significant shift of the peak to larger diameters (p
Original languageEnglish
Pages (from-to)681-686
JournalJournal of Urology
Issue number2
Publication statusPublished - 2011


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