Glycosylated cell surface markers for the isolation of human cardiac progenitors

A.T. Moerkamp, H.W. Leung, N.A.M. Bax, S. Holst, K. Lodder, T. Berends, C.K.E. Dingenouts, A. Choo, A.M. Smits, M.J. Goumans

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Abstract

The aim of stem cell therapy after cardiac injury is to replace damaged cardiac tissue. Human cardiac progenitor cells (CPCs) represent an interesting cell population for clinical strategies to treat cardiac disease and human CPC-specific antibodies would aid in the clinical implementation of cardiac progenitor-based cell therapy. However, the field of CPC biology suffers from the lack of human CPC-specific markers. Therefore, we raised a panel of monoclonal antibodies (mAb) against CPCs. Of this panel of antibodies, we show that mAb C1096 recognizes a progenitor-like population in the fetal and adult human heart and partially colocalize with reported CPC populations in vitro. Furthermore, mAb C1096 can be used to isolate a multipotent progenitor population from human heart tissue. Interestingly, the two lead candidates, mAb C1096 and mAb C19, recognize glycosylated residues on PECAM1 (platelet and endothelial cell adhesion molecule 1) and GRP78, respectively, and de-N-glycosylation significantly abolishes their binding. Thereby, this report describes new clinically applicable antibodies against human CPCs, and for the first time demonstrates the importance of glycosylated residues as CPCs specific markers.

Original languageEnglish
Pages (from-to)1552-1565
Number of pages14
JournalStem Cells and Development
Volume26
Issue number21
DOIs
Publication statusPublished - 1 Nov 2017

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Keywords

  • glycosylation
  • GRP78
  • human cardiac progenitor cells
  • monoclonal antibodies
  • multipotent
  • PECAM1

Cite this

Moerkamp, A. T., Leung, H. W., Bax, N. A. M., Holst, S., Lodder, K., Berends, T., ... Goumans, M. J. (2017). Glycosylated cell surface markers for the isolation of human cardiac progenitors. Stem Cells and Development, 26(21), 1552-1565. https://doi.org/10.1089/scd.2017.0048