GC-MS analysis of ifosfamide

H. Lambrechts, G.G.O. Pattyn, K.A. Cauwenberghe, van, P.A. Leclercq, E.A. Bruijn, de

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Abstract

Ifosfamide (IF) is not subjected to thermal degradation during capillary GC, in contrast to its structural isomer cyclophosphamide (CY). Both 2.65 pm and 0.32 /1m OV-l columns proved to be useful in gas chromatographic analysis of IF without any derivatization. Mass spectrometry data demonstrated that intact IF was eluted while no hindrance of naturally occuring metabolites such as 2-dechloroethyl-IF, 3-dechloroethyl-IF, 4-keto-lF and iso-phosphoramide mustard was found. Therefore, IF can be monitored in biological fluids of cancer patients treated with the oxazaphosphorlne by capillary GC-MS without derivatizatlon. A simple liquid-liquid extraction is required prior to introduction of IF into GC. A single peak of IF is detected with molecular mass 260; fragmentation starts with loss of CH2CI: m/z 211 = IF-CH2CI. The limit of determination of IF in human biological nuids was approximately 10 ng ml-t (50 nM). Recovery, quality of calibration curves, reproducibility, withinrun and within-assay precision allowed rapid determination of IF in a range of 0.01-1000 mug ml-1. Data of GC-MS analysis proved that unchanged IF can be determined in plasma and urine extracts without in situ formation of degradation products, during capillary GC.
Original languageEnglish
Title of host publicationThirteenth International Symposium on Capillary Chromatography, Riva del Garda, Italy, May 13 - 16, 1991
EditorsP.J.F. Sandra
Place of PublicationHeidelberg
PublisherHuethig
Pages1109-1116
Volume2
Publication statusPublished - 1991
Event13th International Symposium on Capillary Chromatography - Palazzo del Congressi, Riva del Garda, Italy
Duration: 13 May 199116 May 1991

Conference

Conference13th International Symposium on Capillary Chromatography
CountryItaly
CityRiva del Garda
Period13/05/9116/05/91

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