Abstract
The cancerous inhibitor of protein phosphatase 2A (CIP2A) is an oncoprotein found overexpressed in many types of cancer. CIP2A has been shown to stabilize oncoproteins such as cMYC by shielding them from PP2A-mediated dephosphorylation. Here we report that the penultimate residue Ser904 in the C-terminus of CIP2A can be phosphorylated to create a binding site for the regulatory protein 14-3-3. We demonstrate that 14-3-3 is a new interaction partner of CIP2A. The 14-3-3/CIP2A C-terminal interaction complex can be targeted by the protein-protein interaction (PPI) stabilizer fusicoccin-A (FC-A), resulting in enhanced levels of phosphorylated Ser904. FC-A treatment of TNBC cells leads to the increased association of CIP2A with 14-3-3. We show that the composite interface between 14 and 3-3 and CIP2A's C-terminus can be targeted by the PPI stabilizer FC-A, providing a new interface that could potentially be exploited to modulate CIP2A's activity.
| Original language | English |
|---|---|
| Pages (from-to) | 2972-2978 |
| Number of pages | 7 |
| Journal | ACS Chemical Biology |
| Volume | 17 |
| Issue number | 11 |
| DOIs | |
| Publication status | Published - 18 Nov 2022 |
Bibliographical note
Funding Information:We want to thank J. Bebelman for assisting with the experimental work. The research described was funded by the VU University Amsterdam and the Amsterdam Institute of Molecules, Medicines and Systems (AIMMS), grant 2016/004.
Funding
We want to thank J. Bebelman for assisting with the experimental work. The research described was funded by the VU University Amsterdam and the Amsterdam Institute of Molecules, Medicines and Systems (AIMMS), grant 2016/004.
