From fiber curls to mesh waves: A platform for fabrication of hierarchically structured nanofibers mimicking natural tissue formation

Honglin Chen, Danielle Batista, Giuseppe Criscenti, João F. Crispim, Hugo Machado Fernandes, Clemens van Blitterswijk, Roman Truckenmuller (Corresponding author), Lorenzo Moroni (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Bioinstructive scaffolds for regenerative medicine are characterized by intrinsic properties capable of directing cell response and promoting wound healing. The design of such scaffolds requires the incorporation of well-defined physical properties that mimic the native extracellular matrix (ECM). Here, inspired by epithelial tissue morphogenesis, we present a novel approach to code nanofiber materials with controlled hierarchical wavy structures resembling the configurations of native EMC fibers through using thermally shrinking materials as substrates onto which the fibers are deposited. This approach could serve as a platform for fabricating functional scaffolds mimicking various tissues such as trachea, iris, artery wall and ciliary body. Modeling affirms that the mechanical properties of the fabricated wavy fibers could be regulated through varying their wavy patterns. The nanofibrous scaffolds coded with wavy patterns show an enhanced cellular infiltration. In addition, we further investigated whether the wavy patterns could regulate transforming growth factor-beta (TGF-β) production, a key signalling pathway involved in connective tissue development. Our results demonstrated that nanofibrous scaffolds coded with wavy patterns could induce TGF-β expression without the addition of a soluble growth factor. Our new approach could open up new avenues for fabricating bioinstructive scaffolds for regenerative medicine.

Original languageEnglish
Pages (from-to)14312-14321
Number of pages10
JournalNanoscale
Volume11
Issue number30
DOIs
Publication statusPublished - 14 Aug 2019

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Nanofibers
Scaffolds
Tissue
Fabrication
Fibers
Transforming Growth Factor beta
Electromagnetic compatibility
Infiltration
Intercellular Signaling Peptides and Proteins
Physical properties
Mechanical properties
Substrates

Cite this

Chen, Honglin ; Batista, Danielle ; Criscenti, Giuseppe ; Crispim, João F. ; Fernandes, Hugo Machado ; van Blitterswijk, Clemens ; Truckenmuller, Roman ; Moroni, Lorenzo. / From fiber curls to mesh waves : A platform for fabrication of hierarchically structured nanofibers mimicking natural tissue formation. In: Nanoscale. 2019 ; Vol. 11, No. 30. pp. 14312-14321.
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Chen, H, Batista, D, Criscenti, G, Crispim, JF, Fernandes, HM, van Blitterswijk, C, Truckenmuller, R & Moroni, L 2019, 'From fiber curls to mesh waves: A platform for fabrication of hierarchically structured nanofibers mimicking natural tissue formation', Nanoscale, vol. 11, no. 30, pp. 14312-14321. https://doi.org/10.1039/C8NR10108F

From fiber curls to mesh waves : A platform for fabrication of hierarchically structured nanofibers mimicking natural tissue formation. / Chen, Honglin; Batista, Danielle; Criscenti, Giuseppe; Crispim, João F.; Fernandes, Hugo Machado; van Blitterswijk, Clemens; Truckenmuller, Roman (Corresponding author); Moroni, Lorenzo (Corresponding author).

In: Nanoscale, Vol. 11, No. 30, 14.08.2019, p. 14312-14321.

Research output: Contribution to journalArticleAcademicpeer-review

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