TY - JOUR
T1 - Fluorescent imaging of transition metal homeostasis using genetically encoded sensors
AU - Vinkenborg, J.L.
AU - Koay, M.S.T.
AU - Merkx, M.
PY - 2010
Y1 - 2010
N2 - The ability to image the concentration of transition metals in living cells in real time is important for understanding transition metal (TM) homeostasis and its involvement in diseases. Genetically encoded fluorescent sensor proteins are attractive because they do not require cell-invasive procedures, can be targeted to different locations in the cell, and allow ratiometric detection. Important progress in the development of Zn2+ sensors has allowed sensitive detection of the very low free concentrations of Zn2+ in single cells, both in the cytosol and various organelles. Together with other recent advances in chemical biology, these tools seem particularly useful to interrogate the dynamics and compartmentation of TM homeostasis.
AB - The ability to image the concentration of transition metals in living cells in real time is important for understanding transition metal (TM) homeostasis and its involvement in diseases. Genetically encoded fluorescent sensor proteins are attractive because they do not require cell-invasive procedures, can be targeted to different locations in the cell, and allow ratiometric detection. Important progress in the development of Zn2+ sensors has allowed sensitive detection of the very low free concentrations of Zn2+ in single cells, both in the cytosol and various organelles. Together with other recent advances in chemical biology, these tools seem particularly useful to interrogate the dynamics and compartmentation of TM homeostasis.
U2 - 10.1016/j.cbpa.2009.11.022
DO - 10.1016/j.cbpa.2009.11.022
M3 - Article
C2 - 20036601
SN - 1367-5931
VL - 14
SP - 231
EP - 237
JO - Current Opinion in Chemical Biology
JF - Current Opinion in Chemical Biology
IS - 2
ER -