Fibrin as a cell carrier in cardiovascular tissue engineering applications

A. Driessen - Mol, M.I. Lieshout, van, G.C. Dam - de Veen, S. Neuenschwander, S.P. Hoerstrup, F.P.T. Baaijens, C.V.C. Bouten

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Abstract

In cardiovascular tissue engineering approaches, efficient seeding methods are essential. To achieve this and to save time, cells can be encapsulated in gels. Combining the advantages of a gel as a cell carrier with the advantages of a fiber-based scaffold, providing structural integrity to the developing tissue, might offer several advantages. In this study, seeding by using fibrin as a cell carrier is compared to the conventional static seeding method with regard to tissue development. Seeding with fibrin resulted in less loss of soluble collagen into the medium and a more mature extracellular matrix in a shorter period of time. The use of fibrin degradation inhibitors was shown to inhibit extracellular matrix formation, although it did not hamper cell proliferation. The use of fibrin as a cell carrier to seed cells into a fiber-based scaffold may represent a promising, timesaving approach in cardiovascular tissue engineering applications.
Original languageEnglish
Pages (from-to)3113-3121
JournalBiomaterials
Volume26
Issue number16
DOIs
Publication statusPublished - 2005

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