Fast high-throughput screening of temoporfin-loaded liposomal formulations prepared by ethanol injection method

K.W. Yang, J.T. Delaney, U.S. Schubert, A. Fahr

Research output: Contribution to journalArticleAcademicpeer-review

20 Citations (Scopus)

Abstract

A new strategy for fast, convenient high-throughput screening of liposomal formulations was developed, utilizing the automation of the so-called ethanol-injection method. This strategy was illustrated by the preparation and screening of the liposomal formulation library of a potent second-generation photosensitizer, temoporfin. Numerous liposomal formulations were efficiently prepared using a pipetting robot, followed by automated size characterization, using a dynamic light scattering plate reader. Incorporation efficiency of temoporfin and zeta potential were also detected in selected cases. To optimize the formulation, different parameters were investigated, including lipid types, lipid concentration in injected ethanol, ratio of ethanol to aqueous solution, ratio of drug to lipid, and the addition of functional phospholipid. Step-by-step small liposomes were prepared with high incorporation efficiency. At last, an optimized formulation was obtained for each lipid in the following condition: 36.4 mg.mL(-1) lipid, 13.1 mg.mL(-1) mPEG(2000)-DSPE, and 1:4 ethanol: buffer ratio. These liposomes were unilamellar spheres, with a diameter of approximately 50 nm, and were very stable for over 20 weeks. The results illustrate this approach to be promising for fast high-throughput screening of liposomal formulations
Original languageEnglish
Pages (from-to)31-41
Number of pages11
JournalJournal of Liposome Research
Volume22
Issue number1
DOIs
Publication statusPublished - 2012

Fingerprint

Dive into the research topics of 'Fast high-throughput screening of temoporfin-loaded liposomal formulations prepared by ethanol injection method'. Together they form a unique fingerprint.

Cite this