Exploiting biased reptation for continuous flow preparative DNA fractionation in a versatile microfluidic platform

Burcu Gumuscu, Johan G. Bomer, Hans L. de Boer, Albert van den Berg, Jan C.T. Eijkel

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

A new approach is presented for preparative, continuous flow fractionation of sub-10-kbp DNA fragments, which exploits the variation in the field-dependent mobility of the DNA molecules based on their length. Orthogonally pulsed electric fields of significantly different magnitudes are applied to a microchip filled with a sieving matrix of 1.2% agarose gel. Using this method, we demonstrate a high-resolution separation of 0.5, 1, 2, 5, and 10 kbp DNA fragments within 2 min. During the separation, DNA fragments are also purified from other ionic species. Preparative fractionation of sub-10-kbp DNA molecules plays an important role in second-generation sequencing. The presented device performs rapid high-resolution fractionation and it can be reliably manufactured with simple microfabrication procedures.

Original languageEnglish
Article number17001
Pages (from-to)1-8
JournalMicrosystems & Nanoengineering
Volume3
DOIs
Publication statusPublished - 22 May 2017
Externally publishedYes

Keywords

  • Agarose gel
  • Biased reptation
  • Continuous flow DNA separation
  • DNA purification
  • Preparative fractionation

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