Experimental reconstruction of an abdominal wall defect with electrospun polycaprolactone-ureidopyrimidinone mesh conserves compliance yet may have insufficient strength

Lucie Hympanova, Marina Gabriela Monteiro Carvalho Mori da Cunha, Rita Rynkevic, Radoslaw A. Wach, Alicja K. Olejnik, Patricia Y.W. Dankers, Boris Arts, Tristan Mes, Anton W. Bosman, Maarten Albersen, Jan Deprest

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Abstract

Purpose: Electrospun meshes mimic the extracellular matrix, which may improve their integration. We aimed to compare polycaprolactone (PCL) modified with ureidopyrimidinone (UPy) electrospun meshes with ultra-lightweight polypropylene (PP; Restorelle) reference textile meshes for in vivo compliance. We chose UPy-PCL because we have shown it does not compromise biomechanical properties of native tissue, and because it potentially can be bioactivated. Methods: We performed ex vivo biomechanical cyclic loading in wet conditions and in vivo overlay of full-thickness abdominal wall defects in rats and rabbits. Animals were sacrificed at 7, 42 and 54 days (rats; n = 6/group) and 30 and 90 days (rabbits; n = 3/group). Outcomes were herniation, mesh degradation and mesh dimensions, explant compliance and histology. High failure rates prompted us to provide additional material strength by increasing fiber diameter and mesh thickness, which was further tested in rabbits as a biomechanically more challenging model. Results: Compliance was tested in animals without herniation. In both species, UPy-PCL-explants were as compliant as native tissue. In rats, PP-explants were stiffer. Contraction was similar in UPy-PCL and PP-explants. However, UPy-PCL-meshes macroscopically degraded from 30 days onwards, coinciding with herniation in up to half of animals. Increased fiber and mesh thickness did not improve outcome. Degradation of UPy-PCL is associated with an abundance of foreign body giant cells until UPy-PCL disappears. Conclusion: Abdominal wall reconstruction with electrospun UPy-PCL meshes failed in 50%. Degradation coincided with a transient vigorous foreign body reaction. Non-failing UPy-PCL-explants were as compliant as native tissue. Despite that, the high failure rate forces us to explore electrospun meshes based on other polymers.

Original languageEnglish
Pages (from-to)431-441
Number of pages11
JournalJournal of the Mechanical Behavior of Biomedical Materials
Volume88
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

Polycaprolactone
Defects
Rats
Animals
Tissue
Degradation
Compliance
polycaprolactone
Histology
Fibers
Polypropylenes
Textiles
Polymers

Keywords

  • Biocompatibility
  • Biomechanics
  • Electrospun mesh
  • Hernia
  • Polypropylene

Cite this

Hympanova, Lucie ; Mori da Cunha, Marina Gabriela Monteiro Carvalho ; Rynkevic, Rita ; Wach, Radoslaw A. ; Olejnik, Alicja K. ; Dankers, Patricia Y.W. ; Arts, Boris ; Mes, Tristan ; Bosman, Anton W. ; Albersen, Maarten ; Deprest, Jan. / Experimental reconstruction of an abdominal wall defect with electrospun polycaprolactone-ureidopyrimidinone mesh conserves compliance yet may have insufficient strength. In: Journal of the Mechanical Behavior of Biomedical Materials. 2018 ; Vol. 88. pp. 431-441.
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title = "Experimental reconstruction of an abdominal wall defect with electrospun polycaprolactone-ureidopyrimidinone mesh conserves compliance yet may have insufficient strength",
abstract = "Purpose: Electrospun meshes mimic the extracellular matrix, which may improve their integration. We aimed to compare polycaprolactone (PCL) modified with ureidopyrimidinone (UPy) electrospun meshes with ultra-lightweight polypropylene (PP; Restorelle) reference textile meshes for in vivo compliance. We chose UPy-PCL because we have shown it does not compromise biomechanical properties of native tissue, and because it potentially can be bioactivated. Methods: We performed ex vivo biomechanical cyclic loading in wet conditions and in vivo overlay of full-thickness abdominal wall defects in rats and rabbits. Animals were sacrificed at 7, 42 and 54 days (rats; n = 6/group) and 30 and 90 days (rabbits; n = 3/group). Outcomes were herniation, mesh degradation and mesh dimensions, explant compliance and histology. High failure rates prompted us to provide additional material strength by increasing fiber diameter and mesh thickness, which was further tested in rabbits as a biomechanically more challenging model. Results: Compliance was tested in animals without herniation. In both species, UPy-PCL-explants were as compliant as native tissue. In rats, PP-explants were stiffer. Contraction was similar in UPy-PCL and PP-explants. However, UPy-PCL-meshes macroscopically degraded from 30 days onwards, coinciding with herniation in up to half of animals. Increased fiber and mesh thickness did not improve outcome. Degradation of UPy-PCL is associated with an abundance of foreign body giant cells until UPy-PCL disappears. Conclusion: Abdominal wall reconstruction with electrospun UPy-PCL meshes failed in 50{\%}. Degradation coincided with a transient vigorous foreign body reaction. Non-failing UPy-PCL-explants were as compliant as native tissue. Despite that, the high failure rate forces us to explore electrospun meshes based on other polymers.",
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author = "Lucie Hympanova and {Mori da Cunha}, {Marina Gabriela Monteiro Carvalho} and Rita Rynkevic and Wach, {Radoslaw A.} and Olejnik, {Alicja K.} and Dankers, {Patricia Y.W.} and Boris Arts and Tristan Mes and Bosman, {Anton W.} and Maarten Albersen and Jan Deprest",
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Experimental reconstruction of an abdominal wall defect with electrospun polycaprolactone-ureidopyrimidinone mesh conserves compliance yet may have insufficient strength. / Hympanova, Lucie; Mori da Cunha, Marina Gabriela Monteiro Carvalho; Rynkevic, Rita; Wach, Radoslaw A.; Olejnik, Alicja K.; Dankers, Patricia Y.W.; Arts, Boris; Mes, Tristan; Bosman, Anton W.; Albersen, Maarten; Deprest, Jan.

In: Journal of the Mechanical Behavior of Biomedical Materials, Vol. 88, 01.12.2018, p. 431-441.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Experimental reconstruction of an abdominal wall defect with electrospun polycaprolactone-ureidopyrimidinone mesh conserves compliance yet may have insufficient strength

AU - Hympanova, Lucie

AU - Mori da Cunha, Marina Gabriela Monteiro Carvalho

AU - Rynkevic, Rita

AU - Wach, Radoslaw A.

AU - Olejnik, Alicja K.

AU - Dankers, Patricia Y.W.

AU - Arts, Boris

AU - Mes, Tristan

AU - Bosman, Anton W.

AU - Albersen, Maarten

AU - Deprest, Jan

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Purpose: Electrospun meshes mimic the extracellular matrix, which may improve their integration. We aimed to compare polycaprolactone (PCL) modified with ureidopyrimidinone (UPy) electrospun meshes with ultra-lightweight polypropylene (PP; Restorelle) reference textile meshes for in vivo compliance. We chose UPy-PCL because we have shown it does not compromise biomechanical properties of native tissue, and because it potentially can be bioactivated. Methods: We performed ex vivo biomechanical cyclic loading in wet conditions and in vivo overlay of full-thickness abdominal wall defects in rats and rabbits. Animals were sacrificed at 7, 42 and 54 days (rats; n = 6/group) and 30 and 90 days (rabbits; n = 3/group). Outcomes were herniation, mesh degradation and mesh dimensions, explant compliance and histology. High failure rates prompted us to provide additional material strength by increasing fiber diameter and mesh thickness, which was further tested in rabbits as a biomechanically more challenging model. Results: Compliance was tested in animals without herniation. In both species, UPy-PCL-explants were as compliant as native tissue. In rats, PP-explants were stiffer. Contraction was similar in UPy-PCL and PP-explants. However, UPy-PCL-meshes macroscopically degraded from 30 days onwards, coinciding with herniation in up to half of animals. Increased fiber and mesh thickness did not improve outcome. Degradation of UPy-PCL is associated with an abundance of foreign body giant cells until UPy-PCL disappears. Conclusion: Abdominal wall reconstruction with electrospun UPy-PCL meshes failed in 50%. Degradation coincided with a transient vigorous foreign body reaction. Non-failing UPy-PCL-explants were as compliant as native tissue. Despite that, the high failure rate forces us to explore electrospun meshes based on other polymers.

AB - Purpose: Electrospun meshes mimic the extracellular matrix, which may improve their integration. We aimed to compare polycaprolactone (PCL) modified with ureidopyrimidinone (UPy) electrospun meshes with ultra-lightweight polypropylene (PP; Restorelle) reference textile meshes for in vivo compliance. We chose UPy-PCL because we have shown it does not compromise biomechanical properties of native tissue, and because it potentially can be bioactivated. Methods: We performed ex vivo biomechanical cyclic loading in wet conditions and in vivo overlay of full-thickness abdominal wall defects in rats and rabbits. Animals were sacrificed at 7, 42 and 54 days (rats; n = 6/group) and 30 and 90 days (rabbits; n = 3/group). Outcomes were herniation, mesh degradation and mesh dimensions, explant compliance and histology. High failure rates prompted us to provide additional material strength by increasing fiber diameter and mesh thickness, which was further tested in rabbits as a biomechanically more challenging model. Results: Compliance was tested in animals without herniation. In both species, UPy-PCL-explants were as compliant as native tissue. In rats, PP-explants were stiffer. Contraction was similar in UPy-PCL and PP-explants. However, UPy-PCL-meshes macroscopically degraded from 30 days onwards, coinciding with herniation in up to half of animals. Increased fiber and mesh thickness did not improve outcome. Degradation of UPy-PCL is associated with an abundance of foreign body giant cells until UPy-PCL disappears. Conclusion: Abdominal wall reconstruction with electrospun UPy-PCL meshes failed in 50%. Degradation coincided with a transient vigorous foreign body reaction. Non-failing UPy-PCL-explants were as compliant as native tissue. Despite that, the high failure rate forces us to explore electrospun meshes based on other polymers.

KW - Biocompatibility

KW - Biomechanics

KW - Electrospun mesh

KW - Hernia

KW - Polypropylene

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U2 - 10.1016/j.jmbbm.2018.08.026

DO - 10.1016/j.jmbbm.2018.08.026

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JO - Journal of the Mechanical Behavior of Biomedical Materials

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SN - 1751-6161

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