Expansion of the assembly of cowpea chlorotic mottle virus towards non-native and physiological conditions

L. Schoonen, R.J.M. Maas, R.J.M. Nolte, J.C.M. van Hest

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Abstract

The cowpea chlorotic mottle virus (CCMV) is a nanoparticle that holds promise for diagnostic and therapeutic applications. The empty virus-like particle, however, is not stable under physiological conditions. Here, we describe a systematic study into the expansion of the assembly properties of a protein based block copolymer of the CCMV capsid protein and an elastin-like polypeptide. By systematically changing the hydrophobicity of the stimulus-responsive elastin-like polypeptide block, assembly of the capsid proteins could be achieved at close to physiological conditions. This strategy may prove to be useful in the development of a physiologically stable CCMV capsid variant. (C) 2017 Elsevier Ltd. All rights reserved.
Original languageEnglish
Article number33
Pages (from-to)4968-4971
Number of pages4
JournalTetrahedron
Volume73
Issue number33
DOIs
Publication statusPublished - 17 Aug 2017

Keywords

  • Cowpea chlorotic mottle virus
  • Elastin-like polypeptide
  • Self-assembly
  • Viral capsid

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