The cowpea chlorotic mottle virus (CCMV) is a nanoparticle that holds promise for diagnostic and therapeutic applications. The empty virus-like particle, however, is not stable under physiological conditions. Here, we describe a systematic study into the expansion of the assembly properties of a protein based block copolymer of the CCMV capsid protein and an elastin-like polypeptide. By systematically changing the hydrophobicity of the stimulus-responsive elastin-like polypeptide block, assembly of the capsid proteins could be achieved at close to physiological conditions. This strategy may prove to be useful in the development of a physiologically stable CCMV capsid variant. (C) 2017 Elsevier Ltd. All rights reserved.
- Cowpea chlorotic mottle virus
- Elastin-like polypeptide
- Viral capsid
Schoonen, L., Maas, R. J. M., Nolte, R. J. M., & van Hest, J. C. M. (2017). Expansion of the assembly of cowpea chlorotic mottle virus towards non-native and physiological conditions. Tetrahedron, 73(33), 4968-4971 . . https://doi.org/10.1016/j.tet.2017.04.038