The incorporation of amino acids into proteins in vivo is controlled by the amino-acyl-tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans-crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl-tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.
|Number of pages||5|
|Journal||Angewandte Chemie - International Edition|
|Publication status||Published - 16 Jun 2000|
- Amino acids
- Protein expression
- Protein modifications