Expanding the scope of protein biosynthesis by altering the methionyl- tRNA synthetase activity of a bacterial expression host

K.L. Kiick, J.C.M. Hest, van, D.A. Tirrell

Research output: Contribution to journalArticleAcademicpeer-review

99 Citations (Scopus)

Abstract

The incorporation of amino acids into proteins in vivo is controlled by the amino-acyl-tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans-crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl-tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.

Original languageEnglish
Pages (from-to)2148-2152
Number of pages5
JournalAngewandte Chemie - International Edition
Volume39
Issue number12
DOIs
Publication statusPublished - 16 Jun 2000
Externally publishedYes

Keywords

  • Amino acids
  • Biosynthesis
  • Ligases
  • Protein expression
  • Protein modifications

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