Abstract
The incorporation of amino acids into proteins in vivo is controlled by the amino-acyl-tRNA synthetases. The successful incorporation of a nonnatural amino acid, trans-crotylglycine (Tcg), into a protein has now been achieved by increasing the methionyl-tRNA synthetase activity of a bacterial expression host (see scheme). The incorporation of Tcg into proteins creates new opportunities for macromolecular synthesis through genetic engineering, due to the rich chemistry of the olefinic side chain.
Original language | English |
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Pages (from-to) | 2148-2152 |
Number of pages | 5 |
Journal | Angewandte Chemie - International Edition |
Volume | 39 |
Issue number | 12 |
DOIs | |
Publication status | Published - 16 Jun 2000 |
Externally published | Yes |
Keywords
- Amino acids
- Biosynthesis
- Ligases
- Protein expression
- Protein modifications