Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models

Esther Wehrle, Duncan C Tourolle Né Betts, Gisela A Kuhn, Ariane C Scheuren, Sandra Hofmann, Ralph Müller (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Longitudinal in vivo micro-computed tomography (micro-CT) is of interest to non-invasively capture the healing process of individual animals in preclinical fracture healing studies. However, it is not known whether longitudinal imaging itself has an impact on callus formation and remodeling. In this study, a scan group received weekly micro-CT measurements (week 0-6), whereas controls were only scanned post-operatively and at week 5 and 6. Registration of consecutive scans using a branching scheme (bridged vs. unbridged defect) combined with a two-threshold approach enabled assessment of localized bone turnover and mineralization kinetics relevant for monitoring callus remodeling. Weekly micro-CT application did not significantly change any of the assessed callus parameters in the defect and periosteal volumes. This was supported by histomorphometry showing only small amounts of cartilage residuals in both groups, indicating progression towards the end of the healing period. Also, immunohistochemical staining of Sclerostin, previously associated with mediating adverse radiation effects on bone, did not reveal differences between groups. The established longitudinal in vivo micro-CT-based approach allows monitoring of healing phases in mouse femur defect models without significant effects of anesthesia, handling and radiation on callus properties. Therefore, this study supports application of longitudinal in vivo micro-CT for healing-phase-specific monitoring of fracture repair in mice.

Original languageEnglish
Article number17445
Number of pages12
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 25 Nov 2019

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Fracture Healing
Femur
Bony Callus
Tomography
Physiologic Calcification
Bone Remodeling
Radiation Effects
Cartilage
Anesthesia
Radiation
Staining and Labeling
Bone and Bones

Cite this

Wehrle, Esther ; Tourolle Né Betts, Duncan C ; Kuhn, Gisela A ; Scheuren, Ariane C ; Hofmann, Sandra ; Müller, Ralph. / Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Longitudinal in vivo micro-computed tomography (micro-CT) is of interest to non-invasively capture the healing process of individual animals in preclinical fracture healing studies. However, it is not known whether longitudinal imaging itself has an impact on callus formation and remodeling. In this study, a scan group received weekly micro-CT measurements (week 0-6), whereas controls were only scanned post-operatively and at week 5 and 6. Registration of consecutive scans using a branching scheme (bridged vs. unbridged defect) combined with a two-threshold approach enabled assessment of localized bone turnover and mineralization kinetics relevant for monitoring callus remodeling. Weekly micro-CT application did not significantly change any of the assessed callus parameters in the defect and periosteal volumes. This was supported by histomorphometry showing only small amounts of cartilage residuals in both groups, indicating progression towards the end of the healing period. Also, immunohistochemical staining of Sclerostin, previously associated with mediating adverse radiation effects on bone, did not reveal differences between groups. The established longitudinal in vivo micro-CT-based approach allows monitoring of healing phases in mouse femur defect models without significant effects of anesthesia, handling and radiation on callus properties. Therefore, this study supports application of longitudinal in vivo micro-CT for healing-phase-specific monitoring of fracture repair in mice.",
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Evaluation of longitudinal time-lapsed in vivo micro-CT for monitoring fracture healing in mouse femur defect models. / Wehrle, Esther; Tourolle Né Betts, Duncan C; Kuhn, Gisela A; Scheuren, Ariane C; Hofmann, Sandra; Müller, Ralph (Corresponding author).

In: Scientific Reports, Vol. 9, No. 1, 17445, 25.11.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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