TY - JOUR

T1 - Estimating the false discovery rate using nonparametric deconvolution

AU - Wiel, van de, M.A.

AU - Kim, K.I.

PY - 2007

Y1 - 2007

N2 - Given a set of microarray data, the problem is to detect differentially expressed genes, using a false discovery rate (FDR) criterion. As opposed to common procedures in the literature, we do not base the selection criterion on statistical significance only, but also on the effect size. Therefore, we select only those genes that are significantly more differentially expressed than some f-fold (e.g., f= 2). This corresponds to use of an interval null domain for the effect size. Based on a simple error model, we discuss a naive estimator for the FDR, interpreted as the probability that the parameter of interest lies in the null-domain (e.g., µ <log2(2) = 1 ) given that the test statistic exceeds a threshold. We improve the naive estimator by using deconvolution. That is, the density of the parameter of interest is recovered from the data. We study performance of the methods using simulations and real data.

AB - Given a set of microarray data, the problem is to detect differentially expressed genes, using a false discovery rate (FDR) criterion. As opposed to common procedures in the literature, we do not base the selection criterion on statistical significance only, but also on the effect size. Therefore, we select only those genes that are significantly more differentially expressed than some f-fold (e.g., f= 2). This corresponds to use of an interval null domain for the effect size. Based on a simple error model, we discuss a naive estimator for the FDR, interpreted as the probability that the parameter of interest lies in the null-domain (e.g., µ <log2(2) = 1 ) given that the test statistic exceeds a threshold. We improve the naive estimator by using deconvolution. That is, the density of the parameter of interest is recovered from the data. We study performance of the methods using simulations and real data.

U2 - 10.1111/j.1541-0420.2006.00736.x

DO - 10.1111/j.1541-0420.2006.00736.x

M3 - Article

C2 - 17825012

VL - 63

SP - 806

EP - 815

JO - Biometrics

JF - Biometrics

SN - 0006-341X

IS - 3

ER -