TY - JOUR
T1 - Essential environmental cues from the satellite cell niche: Optimizing proliferation and differentiation
AU - Boonen, K.J.M.
AU - Rosaria-Chak, K.Y.
AU - Baaijens, F.P.T.
AU - Schaft, van der, D.W.J.
AU - Post, M.J.
PY - 2009
Y1 - 2009
N2 - The use of muscle progenitor cells (MPCs) for regenerative medicine has been severely compromised by their decreased proliferative and differentiative capacity after being cultured in vitro. We hypothesized the loss of pivotal niche factors to be the cause. Therefore, we investigated the proliferative and differentiative response of passage 0 murine MPCs to varying substrate elasticities and protein coatings and found that proliferation was influenced only by elasticity, whereas differentiation was influenced by both elasticity and protein coating. A stiffness of 21 kPa optimally increased the proliferation of MPCs. Regarding differentiation, we demonstrated that fusion of MPCs into myotubes takes place regardless of elasticity. However, ongoing maturation with cross-striations and contractions occurred only on elasticities higher than 3 kPa. Furthermore, maturation was fastest on poly-D-lysine and laminin coatings. Copyright
AB - The use of muscle progenitor cells (MPCs) for regenerative medicine has been severely compromised by their decreased proliferative and differentiative capacity after being cultured in vitro. We hypothesized the loss of pivotal niche factors to be the cause. Therefore, we investigated the proliferative and differentiative response of passage 0 murine MPCs to varying substrate elasticities and protein coatings and found that proliferation was influenced only by elasticity, whereas differentiation was influenced by both elasticity and protein coating. A stiffness of 21 kPa optimally increased the proliferation of MPCs. Regarding differentiation, we demonstrated that fusion of MPCs into myotubes takes place regardless of elasticity. However, ongoing maturation with cross-striations and contractions occurred only on elasticities higher than 3 kPa. Furthermore, maturation was fastest on poly-D-lysine and laminin coatings. Copyright
U2 - 10.1152/ajpcell.00015.2009
DO - 10.1152/ajpcell.00015.2009
M3 - Article
SN - 0363-6143
VL - 296
SP - C1338-C1345
JO - American Journal of Physiology : Cell Physiology
JF - American Journal of Physiology : Cell Physiology
IS - 6
ER -