Enzymatic enantioselective C-C-bond formation in microreactors

K. Koch, R.J.F. van den Berg, P.J. Nieuwland, R. Wijtmans, H.E. Schoemaker, F.P.J.T. Rutjes, J.C.M. Hest, van

Research output: Contribution to journalArticleAcademicpeer-review

51 Citations (Scopus)

Abstract

We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.

Original languageEnglish
Pages (from-to)1028-1033
Number of pages6
JournalBiotechnology and Bioengineering
Volume99
Issue number4
DOIs
Publication statusPublished - 1 Mar 2008
Externally publishedYes

Keywords

  • Crude lysates
  • Enzyme catalysis
  • Lyases
  • Microreactors
  • Screening

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