Enzymatic enantioselective C-C-bond formation in microreactors

K. Koch; R.J.F. van den Berg; P.J. Nieuwland; R. Wijtmans; H.E. Schoemaker; F.P.J.T. Rutjes; J.C.M. Hest, van

doi:10.1002/bit.21649

Enzymatic enantioselective C-C-bond formation in microreactors

K. Koch, R.J.F. van den Berg, P.J. Nieuwland, R. Wijtmans, H.E. Schoemaker, F.P.J.T. Rutjes, J.C.M. Hest, van

Research output: Contribution to journal › Article › Academic › peer-review

53 Citations (Scopus)

Abstract

We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.

Original language	English
Pages (from-to)	1028-1033
Number of pages	6
Journal	Biotechnology and Bioengineering
Volume	99
Issue number	4
DOIs	https://doi.org/10.1002/bit.21649
Publication status	Published - 1 Mar 2008
Externally published	Yes

Keywords

Crude lysates
Enzyme catalysis
Lyases
Microreactors
Screening

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10.1002/bit.21649

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abstract = "We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.",

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AU - Wijtmans, R.

AU - Schoemaker, H.E.

AU - Rutjes, F.P.J.T.

AU - Hest, van, J.C.M.

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KW - Enzyme catalysis

KW - Lyases

KW - Microreactors

KW - Screening

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Enzymatic enantioselective C-C-bond formation in microreactors

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Keywords

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