Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials

J.W.M. Bree, van, J.J.G. Geysen, A.J.M. Pemen

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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Abstract

The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.
Original languageEnglish
Title of host publicationProceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia
Publication statusPublished - 2012
Eventconference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24 -
Duration: 18 Nov 201224 Nov 2012

Conference

Conferenceconference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24
Period18/11/1224/11/12
OtherScientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia

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Cell Culture Techniques
Cell Proliferation
Culture Media
Melanoma
Electrodes
Equipment and Supplies
In Vitro Techniques

Cite this

Bree, van, J. W. M., Geysen, J. J. G., & Pemen, A. J. M. (2012). Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. In Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia
Bree, van, J.W.M. ; Geysen, J.J.G. ; Pemen, A.J.M. / Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. 2012.
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abstract = "The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.",
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Bree, van, JWM, Geysen, JJG & Pemen, AJM 2012, Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. in Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24, 18/11/12.

Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. / Bree, van, J.W.M.; Geysen, J.J.G.; Pemen, A.J.M.

Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. 2012.

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

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AB - The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.

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BT - Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia

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Bree, van JWM, Geysen JJG, Pemen AJM. Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. In Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. 2012