Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials

J.W.M. Bree, van; J.J.G. Geysen; A.J.M. Pemen

Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials

J.W.M. Bree, van, J.J.G. Geysen, A.J.M. Pemen

Electrical Energy Systems

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

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Abstract

The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.

Original language	English
Title of host publication	Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia
Publication status	Published - 2012
Event	conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24 - Duration: 18 Nov 2012 → 24 Nov 2012

Conference

Conference	conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24
Period	18/11/12 → 24/11/12
Other	Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia

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@inproceedings{5a33125128b943d687d10ce49bd1068b,

title = "Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials",

abstract = "The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.",

author = "{Bree, van}, J.W.M. and J.J.G. Geysen and A.J.M. Pemen",

year = "2012",

language = "English",

booktitle = "Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia",

note = "conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24 ; Conference date: 18-11-2012 Through 24-11-2012",

}

Bree, van, JWM, Geysen, JJG & Pemen, AJM 2012, Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. in Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24, 18/11/12.

Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials. / Bree, van, J.W.M.; Geysen, J.J.G.; Pemen, A.J.M.

Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia. 2012.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Academic › peer-review

TY - GEN

T1 - Enhancing cell proliferation by non-contact nanosecond PEF treatment of cell culture vials

AU - Bree, van, J.W.M.

AU - Geysen, J.J.G.

AU - Pemen, A.J.M.

PY - 2012

Y1 - 2012

N2 - The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.

AB - The applicability of nanosecond pulsed electric fields (nsPEF) has been focused on killing of cells by means of direct contact between the nsPEF electrodes and tissue or liquid, such as in melanoma destruction [1] and sterilization of fluids. Here we present a novel, tabletop device that induces nsPEF over an antenna into their native culture medium and sterile and closed cell culture vials. We show that a single exposure to a train of intense electrical field pulses of nanosecond duration can timulate the rate of cell proliferation in mammalian cell cultures in vitro.

M3 - Conference contribution

BT - Proceedings of the Scientific Workshop on Electroporation Based Technologies and Treatments, 18-24 November 2012, Ljubljana, Slovenia

T2 - conference; Scientific workshop on Electroporation Based Technologies and Treatments, Ljubljana, Slovenia; 2012-11-18; 2012-11-24

Y2 - 18 November 2012 through 24 November 2012

ER -