Engineering genetically encoded FRET sensors

L.H. Lindenburg, M. Merkx

Research output: Contribution to journalArticleAcademicpeer-review

46 Citations (Scopus)
125 Downloads (Pure)

Abstract

Förster Resonance Energy Transfer (FRET) between two fluorescent proteins can be exploited to create fully genetically encoded and thus subcellularly targetable sensors. FRET sensors report changes in energy transfer between a donor and an acceptor fluorescent protein that occur when an attached sensor domain undergoes a change in conformation in response to ligand binding. The design of sensitive FRET sensors remains challenging as there are few generally applicable design rules and each sensor must be optimized anew. In this review we discuss various strategies that address this shortcoming, including rational design approaches that exploit self-associating fluorescent domains and the directed evolution of FRET sensors using high-throughput screening.
Original languageEnglish
Pages (from-to)11691-11713
JournalSensors
Volume14
Issue number7
DOIs
Publication statusPublished - 2014

Fingerprint Dive into the research topics of 'Engineering genetically encoded FRET sensors'. Together they form a unique fingerprint.

Cite this