Engineered protein cages for selective heparin encapsulation

Salla Välimäki; Qing Liu; Lise Schoonen; Daan F.M. Vervoort; Nonappa; Veikko Linko; Roeland J.M. Nolte; Jan C.M. van Hest; Mauri A. Kostiainen

doi:10.1039/d0tb02541k

Engineered protein cages for selective heparin encapsulation

Salla Välimäki
, Qing Liu
, Lise Schoonen
, Daan F.M. Vervoort
, Nonappa
, Veikko Linko
, Roeland J.M. Nolte
, Jan C.M. van Hest
, Mauri A. Kostiainen (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

21 Citations (Scopus)

Abstract

A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.

Original language	English
Pages (from-to)	1272-1276
Number of pages	5
Journal	Journal of Materials Chemistry B
Volume	9
Issue number	5
DOIs	https://doi.org/10.1039/d0tb02541k
Publication status	Published - 7 Feb 2021

Funding

We gratefully thank the Academy of Finland (projects 308578, 303804, 267497), the Jane and Aatos Erkko Foundation, the Sigrid Jusélius Foundation and the Emil Aaltonen Foundation for financial support. We acknowledge the provision of facilities at OtaNano – Nanomicroscopy Center (Aalto-NMC) and Bioeconomy Infrastructure. Support was also received from the European Research Council (ERC Advanced Grant 740295 ENCOPOL to R. J. M. N.) and from the Dutch Ministry of Education, Culture

Funders	Funder number
Ministerie van Onderwijs, Cultuur en Wetenschap
European Union's Horizon 2020 - Research and Innovation Framework Programme	740295
European Union's Horizon 2020 - Research and Innovation Framework Programme
Academy of Finland	303804, 267497, 308578
Ministerie van Onderwijs, Cultuur en Wetenschap	024.001.035

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AU - Liu, Qing

AU - Schoonen, Lise

AU - Vervoort, Daan F.M.

AU - Nonappa,

AU - Linko, Veikko

AU - Nolte, Roeland J.M.

AU - van Hest, Jan C.M.

AU - Kostiainen, Mauri A.

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AB - A heparin-specific binding peptide was conjugated to a cowpea chlorotic mottle virus (CCMV) capsid protein, which was subsequently allowed to encapsulate heparin and form capsid-like protein cages. The encapsulation is specific and the capsid-heparin assemblies display negligible hemolytic activity, indicating proper blood compatibility and promising possibilities for heparin antidote applications.

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Engineered protein cages for selective heparin encapsulation

Abstract

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