Engineered microtissues for real-time characterization of cardiomyocyte function

A.C.C. van Spreeuwel, N.A.M. Bax, J. Foolen, M.A. Borochin, D.W.J. van der Schaft, C.S. Chen, C.V.C. Bouten

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Engineered cardiac tissue models become increasingly important for understanding normal and disease cardiac physiology [1]. Where clinical diagnostic tools usually measure overall function of the heart, cardiac tissue models make it possible to focus on single CMs and their microenvironment. The use of in-vitro cardiac disease models can give more insight in the functionality changes of CMs during disease and thereby speed up the development of new therapies. Therefore, we aim to develop a model for healthy and diseased myocardium to study the effect of diseased microenvironments on the mechanical performance of CMs. The platform consists of 3D engineered microtissues with matrix, CMs and fibroblasts (FBs) on an array of polydimethylsiloxane (PDMS) microposts and allows for real-time characterization of CMs and their surrounding matrix. The design was adapted from Legant et. al. [2] and enables us to measure inhomogeneous tissue forces which may occur if not all cells contract equally. Here we focus on optimization and validation of the platform to measure contraction forces and gain insight in CM mechanical functioning.
Original languageEnglish
Title of host publicationASME 2013 Summer Bioengineering Conference, SBC 2013
Number of pages2
Volume1 A
DOIs
Publication statusPublished - 2013
EventASME 2013 Summer Bioengineering Conference (SBC 2013) - Sunriver, United States
Duration: 26 Jun 201329 Jun 2013
http://www.asmeconferences.org/SBC2013/

Conference

ConferenceASME 2013 Summer Bioengineering Conference (SBC 2013)
Abbreviated titleSBC 2013
CountryUnited States
CitySunriver
Period26/06/1329/06/13
Internet address

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