Electron paramagnetic resonance evidence for a C3' sugar radical in crystalline d(CTCTCGAGAG) X-irradiated at 4 K

M.G. Debije, W.A. Bernhard

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

A neutral sugar radical formed by the net loss of hydrogen from C3' has been identified in crystalline DNA X-irradiated at 4 K. Crystals of duplex d(CTCTCGAGAG), known to be of B conformation, were studied using electron paramagnetic resonance (EPR) spectroscopy. The C3' radical was identified by using information from dose saturation, power saturation, thermal annealing, and spectrum simulation. The yield of the C3' radical, G(C3'), is 0.03 ± 0.01 µmol/J, and its concentration does not appear to saturate up to at least 100 kGy. In the region in which total radical concentration increases linearly with dose, the C3' radical makes up about 4.5% of the total radical population trapped in the oligodeoxynucleotide crystal at 4 K. Based on free base release measured in other oligodeoxynucleotides, we suggest that in d(CTCTCGAGAG) the C3' radical is responsible for about one-third of the strand breakage events.
LanguageEnglish
Pages687-692
Number of pages6
JournalRadiation Research
Volume155
Issue number5
DOIs
StatePublished - 2001

Fingerprint

Oligodeoxyribonucleotides
Electron Spin Resonance Spectroscopy
sugars
electron paramagnetic resonance
Hydrogen
Spectrum Analysis
Hot Temperature
DNA
Population
saturation
dosage
strands
crystals
deoxyribonucleic acid
annealing
hydrogen
spectroscopy

Cite this

@article{e7424322edcc4585abc4561242f25321,
title = "Electron paramagnetic resonance evidence for a C3' sugar radical in crystalline d(CTCTCGAGAG) X-irradiated at 4 K",
abstract = "A neutral sugar radical formed by the net loss of hydrogen from C3' has been identified in crystalline DNA X-irradiated at 4 K. Crystals of duplex d(CTCTCGAGAG), known to be of B conformation, were studied using electron paramagnetic resonance (EPR) spectroscopy. The C3' radical was identified by using information from dose saturation, power saturation, thermal annealing, and spectrum simulation. The yield of the C3' radical, G(C3'), is 0.03 ± 0.01 µmol/J, and its concentration does not appear to saturate up to at least 100 kGy. In the region in which total radical concentration increases linearly with dose, the C3' radical makes up about 4.5{\%} of the total radical population trapped in the oligodeoxynucleotide crystal at 4 K. Based on free base release measured in other oligodeoxynucleotides, we suggest that in d(CTCTCGAGAG) the C3' radical is responsible for about one-third of the strand breakage events.",
author = "M.G. Debije and W.A. Bernhard",
year = "2001",
doi = "10.1667/0033-7587(2001)155[0687:EPREFA]2.0.CO;2",
language = "English",
volume = "155",
pages = "687--692",
journal = "Radiation Research",
issn = "1938-5404",
publisher = "Radiation Research Society",
number = "5",

}

Electron paramagnetic resonance evidence for a C3' sugar radical in crystalline d(CTCTCGAGAG) X-irradiated at 4 K. / Debije, M.G.; Bernhard, W.A.

In: Radiation Research, Vol. 155, No. 5, 2001, p. 687-692.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Electron paramagnetic resonance evidence for a C3' sugar radical in crystalline d(CTCTCGAGAG) X-irradiated at 4 K

AU - Debije,M.G.

AU - Bernhard,W.A.

PY - 2001

Y1 - 2001

N2 - A neutral sugar radical formed by the net loss of hydrogen from C3' has been identified in crystalline DNA X-irradiated at 4 K. Crystals of duplex d(CTCTCGAGAG), known to be of B conformation, were studied using electron paramagnetic resonance (EPR) spectroscopy. The C3' radical was identified by using information from dose saturation, power saturation, thermal annealing, and spectrum simulation. The yield of the C3' radical, G(C3'), is 0.03 ± 0.01 µmol/J, and its concentration does not appear to saturate up to at least 100 kGy. In the region in which total radical concentration increases linearly with dose, the C3' radical makes up about 4.5% of the total radical population trapped in the oligodeoxynucleotide crystal at 4 K. Based on free base release measured in other oligodeoxynucleotides, we suggest that in d(CTCTCGAGAG) the C3' radical is responsible for about one-third of the strand breakage events.

AB - A neutral sugar radical formed by the net loss of hydrogen from C3' has been identified in crystalline DNA X-irradiated at 4 K. Crystals of duplex d(CTCTCGAGAG), known to be of B conformation, were studied using electron paramagnetic resonance (EPR) spectroscopy. The C3' radical was identified by using information from dose saturation, power saturation, thermal annealing, and spectrum simulation. The yield of the C3' radical, G(C3'), is 0.03 ± 0.01 µmol/J, and its concentration does not appear to saturate up to at least 100 kGy. In the region in which total radical concentration increases linearly with dose, the C3' radical makes up about 4.5% of the total radical population trapped in the oligodeoxynucleotide crystal at 4 K. Based on free base release measured in other oligodeoxynucleotides, we suggest that in d(CTCTCGAGAG) the C3' radical is responsible for about one-third of the strand breakage events.

U2 - 10.1667/0033-7587(2001)155[0687:EPREFA]2.0.CO;2

DO - 10.1667/0033-7587(2001)155[0687:EPREFA]2.0.CO;2

M3 - Article

VL - 155

SP - 687

EP - 692

JO - Radiation Research

T2 - Radiation Research

JF - Radiation Research

SN - 1938-5404

IS - 5

ER -