Electrochemical genosensor for the specific detection of SARS-CoV-2

Sebastian Cajigas Bastidas, Daniel Alzate, Maritza Fernández, Carlos Muskus, Jahir Orozco (Corresponding author)

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

Infection caused by Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) is responsible for the Coronavirus disease (COVID-19) and the current pandemic. Its mortality rate increases, demonstrating the imperative need for acute and rapid diagnostic tools as an alternative to current serological tests and molecular techniques. Features of electrochemical genosensor devices make them amenable for fast and accurate testing closer to the patient. This work reports on a specific electrochemical genosensor for SARS-CoV-2 detection and discrimination against homologous respiratory viruses. The electrochemical biosensor was assembled by immobilizing thiolated capture probes on top of maleimide-coated magnetic particles, followed by specific target hybridization between the capture and biotinylated signaling probes in a sandwich-type manner. The probes were rigorously designed bioinformatically and tested in vitro. Enzymatic complexes based on streptavidin-horseradish peroxidase linked the biotinylated signaling probe to render the biosensor electrochemical response. The genosensor showed to reach a sensitivity of 174.4 μA fM−1 and a limit of detection of 807 fM when using streptavidin poly-HRP20 enzymatic complex, detected SARS-CoV-2 specifically and discriminated it against homologous viruses in spiked samples and samples from SARS-CoV-2 cell cultures, a step forward to detect SARS-CoV-2 closer to the patient as a promising way for diagnosis and surveillance of COVID-19.
Original languageEnglish
Article number123482
Number of pages7
JournalTalanta
Volume245
DOIs
Publication statusPublished - 1 Aug 2022
Externally publishedYes

Keywords

  • Genosensor
  • SARS-CoV-2
  • Nanobioconjugate
  • DNA-RNA
  • Pandemics
  • SARS-CoV-2/genetics
  • Biosensing Techniques/methods
  • Humans
  • Streptavidin
  • Electrochemical Techniques/methods
  • COVID-19/diagnosis

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