TY - JOUR
T1 - Efficient differentiation of CD14+ monocytic cells into endothelial cells on degradable biomaterials
AU - Krenning, Guido
AU - Dankers, Patricia Y.W.
AU - Jovanovic, Danijela
AU - van Luyn, Marja J.A.
AU - Harmsen, Martin C.
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Vascular tissue engineering aims at creating self-renewing, anti-thrombogenic, vascular grafts, which can be based on endothelial progenitor cells (EPC). EPC harbor essential features such as plasticity and longevity. Unfortunately, the archetype CD34+ EPC is rare in peripheral blood. Monocytes, i.e. CD14+ cells also have the ability to differentiate into endothelial-like cells and are by far more abundant in peripheral blood than are CD34+ EPC. Therefore, CD14+ cells would seem appropriate candidates for tissue engineering of small-diameter blood vessels. In this study, we investigated the differentiation of CD14+ cells on three biodegradable biomaterials under angiogenic conditions. Morphological analyses, gene transcript analyses, endothelial marker (i.e. VE-Cadherin and eNOS) and macrophage marker (i.e. CD68 and CD163) expression analyses, revealed that a small fraction (15-25%) of cultured CD14+ cells differentiated into macrophages after 21 days of culture. The majority of CD14+ cells (>75%) differentiated into endothelial-like cells (ELC) on all biomaterials used. The expression of endothelial markers was similar to their expression on HUVEC. Since CD14+ cells are present in high numbers in adult peripheral blood, easy to isolate and because they easily differentiate into ELC on biomaterials, we conclude that CD14+ cells are a suitable cell source for progenitor-based vascular tissue engineering.
AB - Vascular tissue engineering aims at creating self-renewing, anti-thrombogenic, vascular grafts, which can be based on endothelial progenitor cells (EPC). EPC harbor essential features such as plasticity and longevity. Unfortunately, the archetype CD34+ EPC is rare in peripheral blood. Monocytes, i.e. CD14+ cells also have the ability to differentiate into endothelial-like cells and are by far more abundant in peripheral blood than are CD34+ EPC. Therefore, CD14+ cells would seem appropriate candidates for tissue engineering of small-diameter blood vessels. In this study, we investigated the differentiation of CD14+ cells on three biodegradable biomaterials under angiogenic conditions. Morphological analyses, gene transcript analyses, endothelial marker (i.e. VE-Cadherin and eNOS) and macrophage marker (i.e. CD68 and CD163) expression analyses, revealed that a small fraction (15-25%) of cultured CD14+ cells differentiated into macrophages after 21 days of culture. The majority of CD14+ cells (>75%) differentiated into endothelial-like cells (ELC) on all biomaterials used. The expression of endothelial markers was similar to their expression on HUVEC. Since CD14+ cells are present in high numbers in adult peripheral blood, easy to isolate and because they easily differentiate into ELC on biomaterials, we conclude that CD14+ cells are a suitable cell source for progenitor-based vascular tissue engineering.
KW - Cell culture
KW - Endothelial cell
KW - Monotype
KW - Polycaprolactone
KW - Polyurethane
KW - RGD peptide
UR - http://www.scopus.com/inward/record.url?scp=33846011899&partnerID=8YFLogxK
U2 - 10.1016/j.biomaterials.2006.11.017
DO - 10.1016/j.biomaterials.2006.11.017
M3 - Article
C2 - 17166584
AN - SCOPUS:33846011899
SN - 0142-9612
VL - 28
SP - 1470
EP - 1479
JO - Biomaterials
JF - Biomaterials
IS - 8
ER -