Effects of temperature and doxorubicin exposure on keratinocyte damage in vitro

F.E.M. Janssen, C.V.C. Bouten, G.M.J. Leeuwen, van, A.A. Steenhoven, van

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Cancer chemotherapy treatment often leads to hair loss, which may be prevented by cooling the scalp during drug administration. The current hypothesis for the hair preservative effect of scalp cooling is that cooling of the scalp skin reduces blood flow (perfusion) and chemical reaction rates. Reduced perfusion leads to less drugs available for uptake, whereas the reduced temperature decreases uptake of and damage by chemotherapy. Altogether, less damage is exerted to the hair cells, and the hair is preserved. However, the two mechanisms in the hypothesis have not been quantified yet. To quantify the effect of reduced drug damage caused by falling temperatures, we investigated the effect of local drug concentration and local tissue temperature on hair cell damage using in vitro experiments on keratinocytes. Cells were exposed for 4 h to a wide range of doxorubicin concentrations. During exposure, cells were kept at different temperatures. Cell viability was determined after 3 d using a viability test. Control samples were used to establish a concentration–viability curve. Results show that cell survival is significantly higher in cooled cells (T
Original languageEnglish
Pages (from-to)81-86
JournalIn Vitro Cellular & Developmental Biology - Animal
Volume44
Issue number3-4
DOIs
Publication statusPublished - 2008

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