Effects of structural modifications on some physical characteristics of exo-polysaccharides from Lactococcus lactis

R. Tuinier; W.H.M. Casteren, van; P.J. Looijesteijn; H.A. Schols; A.G.J. Voragen; P. Zoon

doi:10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-V

Effects of structural modifications on some physical characteristics of exo-polysaccharides from Lactococcus lactis

R. Tuinier, W.H.M. Casteren, van, P.J. Looijesteijn, H.A. Schols, A.G.J. Voragen, P. Zoon

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Abstract

The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs.

Original language	English
Pages (from-to)	160-166
Journal	Biopolymers
Volume	59
DOIs	https://doi.org/10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-V
Publication status	Published - 2001
Externally published	Yes

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title = "Effects of structural modifications on some physical characteristics of exo-polysaccharides from Lactococcus lactis",

abstract = "The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs.",

author = "R. Tuinier and {Casteren, van}, W.H.M. and P.J. Looijesteijn and H.A. Schols and A.G.J. Voragen and P. Zoon",

year = "2001",

doi = "10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-V",

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pages = "160--166",

journal = "Biopolymers",

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TY - JOUR

T1 - Effects of structural modifications on some physical characteristics of exo-polysaccharides from Lactococcus lactis

AU - Tuinier, R.

AU - Casteren, van, W.H.M.

AU - Looijesteijn, P.J.

AU - Schols, H.A.

AU - Voragen, A.G.J.

AU - Zoon, P.

PY - 2001

Y1 - 2001

N2 - The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs.

AB - The relationship between the primary structure and the chain stiffness of exopolysaccharides (EPSs) and modified EPSs produced by two strains of Lactococcus lactis subsp. cremoris was investigated. The molar mass and radius of gyration of these exopolysaccharides were analyzed by multiangle static light scattering after size-exclusion chromatography. From these results and the chemical structure of the repeating units of the investigated EPSs, the Kuhn lengths could be calculated. We found that the initial Kuhn lengths of the two native EPSs are similar. Modification of the EPSs by removing parts of the side groups resulted in a decrease in both the absolute value and the normalized value of the Kuhn length. It is therefore concluded that partial removal of the side groups of these polysaccharides could make them less efficient as thickeners if no specific interaction with other components occurs.

U2 - 10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-V

DO - 10.1002/1097-0282(200109)59:3<160::AID-BIP1015>3.0.CO;2-V

M3 - Article

SN - 0006-3525

VL - 59

SP - 160

EP - 166

JO - Biopolymers

JF - Biopolymers

ER -

Effects of structural modifications on some physical characteristics of exo-polysaccharides from Lactococcus lactis

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