Effects of sample matrix and injection plug on dsDNA migration in capillary gel electrophoresis

M.J. Schans, van der, J.K. Allen, B.J. Wanders, A. Guttman

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Abstract

Reproducible DNA migration times are required for accurate basepair assignment in restriction fragment mapping and polymerase chain reaction product identification. This data shows DNA migration time shifts with changes in sample ionic strength. Secondly, loss of resoln. with replaceable polyacrylamide gels was obsd. when increasing the length of the sample plug with pressure injection. An easy way to correct for the migration time shifts is to incorporate an internal DNA std. directly into the sepn. process by consecutively injecting the DNA sample and the DNA std. This allows for compensation of any possible migration time variation caused by high ionic strength sample matrixes. Also high-resoln. sepns. can be maintained with large injection vols. (long injection plug) by using consecutive injections of 0.1 M Tris-acetate buffer and the DNA sample. [on SciFinder (R)]
Original languageEnglish
Pages (from-to)511-516
JournalJournal of Chromatography, A
Volume680
Issue number2
DOIs
Publication statusPublished - 1994

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