Effect of oxygen upon the microdetermination of histidine with the aid of the Pauly reaction

L.A.A.E. Sluyterman

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

The colour obtained upon the addition of diazosulphanilic acid to histidine in alkaline medium (Pauly reaction) is bleached rather suddenly after a certain lag. This lag is shorter the more oxygen is present in the alkaline reaction medium. A method of determining histidine on a micro scale, consisting of an improved Pauly reaction after paperchromatographic separation, is described in detail.
Original languageEnglish
Pages (from-to)218-221
Number of pages4
JournalBiochimica et Biophysica Acta
Volume38
DOIs
Publication statusPublished - 1960

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