Effect of chordin-like 1 on MC3T3-E1 and human mesenchymal stem cells

Hugo Fernandes, Koen Dechering, Eugene Van Someren, Ilse Steeghs, Marion Apotheker, Anouk Mentink, Clemens Van Blitterswijk, Jan De Boer

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Since the discovery that bone morphogenetic proteins (BMPs) are able to induce ectopic bone formation, considerable effort has been devoted to apply it for bone regeneration. BMP activity needs to be temporally and spatially controlled and the organism has devised ways to achieve it. Here we show that the BMP inhibitor chordin-like 1 can interfere with BMP2 signalling thereby affecting the osteogenic differentiation of MC3T3-E1 cells. Besides its function as a BMP antagonist, chordin-like 1 enhanced the proliferation of human mesenchymal stem cells (hMSCs) in a BMP2-independent manner. When MC3T3-E1 cells were exposed to recombinant chordin-like 1 there was an inhibition of alkaline phosphatase (ALP) expression, whereas in the case of hMSCs no effect was observed. However, chordin-like 1 dose-dependently increased the proliferation of hMSCs. This effect is probably BMP2 independent because the chordin-like 1 concentration that stimulates proliferation does not interfere with BMP signalling monitored by a Smad-dependent reporter gene. Our data point towards a novel, BMP-independent role of chordin-like 1 in hMSC proliferation.

Original languageEnglish
Pages (from-to)443-452
Number of pages10
JournalCells Tissues Organs
Volume191
Issue number6
DOIs
Publication statusPublished - 1 May 2010
Externally publishedYes

Keywords

  • BMP2
  • Bone morphogenetic protein
  • Bone regeneration
  • Chordin-like 1
  • Mesenchymal stem cells
  • Proliferation

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