Dynamic modulation of proximity-induced enzyme activity using supramolecular polymers

Eva Magdalena Estirado; Bas J H M Rosier; Tom F A de Greef; Luc Brunsveld

doi:10.1039/d0cc02120b

Dynamic modulation of proximity-induced enzyme activity using supramolecular polymers

Eva Magdalena Estirado
, Bas J H M Rosier
, Tom F A de Greef
, Luc Brunsveld (Corresponding author)

Research output: Contribution to journal › Article › Academic › peer-review

4 Citations (Scopus)

Abstract

Synthetic supramolecular polymers are used as dynamic nanoscaffolds for the activation of the apoptotic signalling enzyme caspase-9. Recruitment of caspase-9 to the nanoscaffold results in an increase in enzymatic activity due to enhanced proximity, with a bell-shaped response as a function of nanoscaffold concentration. The modularity of the system allows for dynamic regulation of enzyme activity through variation of the recruitment-motif density along the supramolecular polymer.

Original language	English
Pages (from-to)	5747-5750
Number of pages	4
Journal	Chemical Communications, ChemComm
Volume	56
Issue number	43
Early online date	22 Apr 2020
DOIs	https://doi.org/10.1039/d0cc02120b
Publication status	Published - 28 May 2020

Funding

This work was supported by The Netherlands Organization for Scientific Research (NWO) through Gravity Program 024.001.035, and through the European Union’s program Horizon 2020 Marie Curie Innovative Training Network MULTI APP (2015-2019).

Funders	Funder number
Nederlandse Organisatie voor Wetenschappelijk Onderzoek
European Union's Horizon 2020 - Research and Innovation Framework Programme	677313
Nederlandse Organisatie voor Wetenschappelijk Onderzoek	024.001.035
European Union's Horizon 2020 - Research and Innovation Framework Programme	2015-2019

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10.1039/d0cc02120b

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abstract = "Synthetic supramolecular polymers are used as dynamic nanoscaffolds for the activation of the apoptotic signalling enzyme caspase-9. Recruitment of caspase-9 to the nanoscaffold results in an increase in enzymatic activity due to enhanced proximity, with a bell-shaped response as a function of nanoscaffold concentration. The modularity of the system allows for dynamic regulation of enzyme activity through variation of the recruitment-motif density along the supramolecular polymer.",

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AU - de Greef, Tom F A

AU - Brunsveld, Luc

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Dynamic modulation of proximity-induced enzyme activity using supramolecular polymers

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