Dynamic and bio-orthogonal protein assembly along a supramolecular polymer

K. Petkau - Milroy, D.A. Uhlenheuer, A.J.H. Spiering, J.A.J.M. Vekemans, L. Brunsveld

Research output: Contribution to journalArticleAcademicpeer-review

30 Citations (Scopus)
124 Downloads (Pure)

Abstract

Dynamic protein assembly along supramolecular columnar polymers has been achieved through the site-specific covalent attachment of different SNAP-tag fusion proteins to self-assembled benzylguanine-decorated discotics. The self-assembly of monovalent discotics into supramolecular polymers creates a multivalent, bio-orthogonal and self-regulating framework for protein assembly. The intrinsic reversibility of supramolecular interactions results in reorganization and exchange of building blocks allowing for dynamic intermixing of protein-functionalized discotics between different self-assembled polymers, leading to self-optimization of protein arrangement and distance as evidenced by efficient energy transfer between fluorescent proteins.
Original languageEnglish
Pages (from-to)2886-2891
Number of pages6
JournalChemical Science
Volume4
Issue number7
DOIs
Publication statusPublished - 2013

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