Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns

Nick R.M. Beijer, Zarina M. Nauryzgaliyeva, Estela M. Arteaga, Laurent Pieuchot, Karine Anselme, Jeroen van de Peppel, Aliaksei S. Vasilevich, Nathalie Groen, Nadia Roumans, Dennie G.A.J. Hebels, Jan de Boer (Corresponding author)

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Abstract

Human mesenchymal stem (hMSCs) are defined as multi-potent colony-forming cells expressing a specific subset of plasma membrane markers when grown on flat tissue culture polystyrene. However, as soon as hMSCs are used for transplantation, they are exposed to a 3D environment, which can strongly impact cell physiology and influence proliferation, differentiation and metabolism. Strategies to control in vivo hMSC behavior, for instance in stem cell transplantation or cancer treatment, are skewed by the un-physiological flatness of the standard well plates. Even though it is common knowledge that cells behave differently in vitro compared to in vivo, only little is known about the underlying adaptation processes. Here, we used micrometer-scale defined surface topographies as a model to describe the phenotype of hMSCs during this adaptation to their new environment. We used well established techniques to compare hMSCs cultured on flat and topographically enhanced polystyreneand observed dramatically changed cell morphologies accompanied by shrinkage of cytoplasm and nucleus, a decreased overall cellular metabolism, and slower cell cycle progression resulting in a lower proliferation rate in cells exposed to surface topographies. We hypothesized that this reduction in proliferation rate effects their sensitivity to certain cancer drugs, which was confirmed by higher survival rate of hMSCs cultured on topographies exposed to paclitaxel. Thus, micro-topographies can be used as a model system to mimic the natural cell micro-environment, and be a powerful tool to optimize cell treatment in vitro.

Original languageEnglish
Article number9099
Number of pages14
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - 1 Dec 2019

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Cell Physiological Phenomena
Mesenchymal Stromal Cells
Polystyrenes
Stem Cell Transplantation
Paclitaxel
Neoplasms
Cell Cycle
Cytoplasm
Transplantation
Cell Membrane
Phenotype
Therapeutics
Pharmaceutical Preparations

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Beijer, N. R. M., Nauryzgaliyeva, Z. M., Arteaga, E. M., Pieuchot, L., Anselme, K., van de Peppel, J., ... Boer, J. D. (2019). Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns. Scientific Reports, 9(1), [9099]. https://doi.org/10.1038/s41598-019-45284-y
Beijer, Nick R.M. ; Nauryzgaliyeva, Zarina M. ; Arteaga, Estela M. ; Pieuchot, Laurent ; Anselme, Karine ; van de Peppel, Jeroen ; Vasilevich, Aliaksei S. ; Groen, Nathalie ; Roumans, Nadia ; Hebels, Dennie G.A.J. ; Boer, Jan de. / Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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Beijer, NRM, Nauryzgaliyeva, ZM, Arteaga, EM, Pieuchot, L, Anselme, K, van de Peppel, J, Vasilevich, AS, Groen, N, Roumans, N, Hebels, DGAJ & Boer, JD 2019, 'Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns', Scientific Reports, vol. 9, no. 1, 9099. https://doi.org/10.1038/s41598-019-45284-y

Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns. / Beijer, Nick R.M.; Nauryzgaliyeva, Zarina M.; Arteaga, Estela M.; Pieuchot, Laurent; Anselme, Karine; van de Peppel, Jeroen; Vasilevich, Aliaksei S.; Groen, Nathalie; Roumans, Nadia; Hebels, Dennie G.A.J.; Boer, Jan de (Corresponding author).

In: Scientific Reports, Vol. 9, No. 1, 9099, 01.12.2019.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Anselme, Karine

AU - van de Peppel, Jeroen

AU - Vasilevich, Aliaksei S.

AU - Groen, Nathalie

AU - Roumans, Nadia

AU - Hebels, Dennie G.A.J.

AU - Boer, Jan de

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Beijer NRM, Nauryzgaliyeva ZM, Arteaga EM, Pieuchot L, Anselme K, van de Peppel J et al. Dynamic adaptation of mesenchymal stem cell physiology upon exposure to surface micropatterns. Scientific Reports. 2019 Dec 1;9(1). 9099. https://doi.org/10.1038/s41598-019-45284-y