Dual-wavelength UV-absorption detection in capillary isotachophoresis

J.C. Reijenga, T.P.E.M. Verheggen, F.M. Everaerts

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Instrumentation for dual-wavelength UV-absorption detection in isotachophoresis is described and evaluated. Computerized signal storage and processing allow data reduction on the basis of the ratio of absorption at any two of the wavelengths 206, 254, 289 and 340 nm. The purity of UV-absorbing spikes or zones is verified by plotting the ratio versus time, the ratio versus one wavelength or one wavelength versus the other. The method is illustrated with the analysis of a nucleotide extract of eggs of Nassarius reticularis
Original languageEnglish
Pages (from-to)75-84
JournalJournal of Chromatography
Issue number1
Publication statusPublished - 1983


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