Dual-wavelength UV-absorption detection in capillary isotachophoresis

J.C. Reijenga; T.P.E.M. Verheggen; F.M. Everaerts

doi:10.1016/S0021-9673(01)90820-9

Dual-wavelength UV-absorption detection in capillary isotachophoresis

J.C. Reijenga, T.P.E.M. Verheggen, F.M. Everaerts

Chemical Engineering and Chemistry

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Abstract

Instrumentation for dual-wavelength UV-absorption detection in isotachophoresis is described and evaluated. Computerized signal storage and processing allow data reduction on the basis of the ratio of absorption at any two of the wavelengths 206, 254, 289 and 340 nm. The purity of UV-absorbing spikes or zones is verified by plotting the ratio versus time, the ratio versus one wavelength or one wavelength versus the other. The method is illustrated with the analysis of a nucleotide extract of eggs of Nassarius reticularis

Original language	English
Pages (from-to)	75-84
Journal	Journal of Chromatography
Volume	267
Issue number	1
DOIs	https://doi.org/10.1016/S0021-9673(01)90820-9
Publication status	Published - 1983

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title = "Dual-wavelength UV-absorption detection in capillary isotachophoresis",

abstract = "Instrumentation for dual-wavelength UV-absorption detection in isotachophoresis is described and evaluated. Computerized signal storage and processing allow data reduction on the basis of the ratio of absorption at any two of the wavelengths 206, 254, 289 and 340 nm. The purity of UV-absorbing spikes or zones is verified by plotting the ratio versus time, the ratio versus one wavelength or one wavelength versus the other. The method is illustrated with the analysis of a nucleotide extract of eggs of Nassarius reticularis",

author = "J.C. Reijenga and T.P.E.M. Verheggen and F.M. Everaerts",

year = "1983",

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AU - Reijenga, J.C.

AU - Verheggen, T.P.E.M.

AU - Everaerts, F.M.

PY - 1983

Y1 - 1983

N2 - Instrumentation for dual-wavelength UV-absorption detection in isotachophoresis is described and evaluated. Computerized signal storage and processing allow data reduction on the basis of the ratio of absorption at any two of the wavelengths 206, 254, 289 and 340 nm. The purity of UV-absorbing spikes or zones is verified by plotting the ratio versus time, the ratio versus one wavelength or one wavelength versus the other. The method is illustrated with the analysis of a nucleotide extract of eggs of Nassarius reticularis

AB - Instrumentation for dual-wavelength UV-absorption detection in isotachophoresis is described and evaluated. Computerized signal storage and processing allow data reduction on the basis of the ratio of absorption at any two of the wavelengths 206, 254, 289 and 340 nm. The purity of UV-absorbing spikes or zones is verified by plotting the ratio versus time, the ratio versus one wavelength or one wavelength versus the other. The method is illustrated with the analysis of a nucleotide extract of eggs of Nassarius reticularis

U2 - 10.1016/S0021-9673(01)90820-9

DO - 10.1016/S0021-9673(01)90820-9

M3 - Article

SN - 0021-9673

VL - 267

SP - 75

EP - 84

JO - Journal of Chromatography

JF - Journal of Chromatography

IS - 1

ER -

Dual-wavelength UV-absorption detection in capillary isotachophoresis

Abstract

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