DNA sequencing with reagent recycling on wiregrid

Lub Johan (Inventor), Jan Van Der Zaag Pieter (Inventor), Wimberger-Friedl Reinhold (Inventor)

Research output: PatentPatent publication

Abstract

The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moietys. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked.

Original languageEnglish
Patent numberWO2013105025
IPCG01N 21/ 64 A I
Priority date9/01/13
Filing date9/01/13
Publication statusPublished - 13 Jun 2017

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