DNA-coatings: Bioactive properties and effects on osteoblast-like cells

J.J.J.P. Beucken, van den; X.F. Walboomers; S.G.G. Leeuwenburgh; M.R.J. Vos; N.A.J.M. Sommerdijk; R.J.M. Nolte; J.A. Jansen

DNA-coatings: Bioactive properties and effects on osteoblast-like cells

J.J.J.P. Beucken, van den, X.F. Walboomers, S.G.G. Leeuwenburgh, M.R.J. Vos, N.A.J.M. Sommerdijk, R.J.M. Nolte, J.A. Jansen

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Abstract

The aim of this study was to evaluate potential effects of DNA-coatings on calcium phosphate (CaP) nucleation from simulated body fluids (SBF) and subsequently the effects of DNA-coatings and SBF-immersed DNA coatings on the behavior of osteoblast-like cells. DNAcoatings demonstrated to enhance the nucleation and deposition of CaP from SBF compared to titanium controls. The behavior of osteoblast-like cells was affected on SBF-immersed DNAcoatings, showing an increased deposition of the extracellular matrix protein osteocalcin compared to titanium controls. These results indicate bone-bonding capacity of DNA-coatings, which needs to be confirmed using future animal experiments.

Original language	English
Pages (from-to)	605-608
Journal	Key Engineering Materials
Volume	361-363
Publication status	Published - 2008

Keywords

DNA-coating
mineral deposition
calcium phosphate
SBF
cell culture

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title = "DNA-coatings: Bioactive properties and effects on osteoblast-like cells",

abstract = "The aim of this study was to evaluate potential effects of DNA-coatings on calcium phosphate (CaP) nucleation from simulated body fluids (SBF) and subsequently the effects of DNA-coatings and SBF-immersed DNA coatings on the behavior of osteoblast-like cells. DNAcoatings demonstrated to enhance the nucleation and deposition of CaP from SBF compared to titanium controls. The behavior of osteoblast-like cells was affected on SBF-immersed DNAcoatings, showing an increased deposition of the extracellular matrix protein osteocalcin compared to titanium controls. These results indicate bone-bonding capacity of DNA-coatings, which needs to be confirmed using future animal experiments.",

keywords = "DNA-coating, mineral deposition, calcium phosphate, SBF, cell culture",

author = "{Beucken, van den}, J.J.J.P. and X.F. Walboomers and S.G.G. Leeuwenburgh and M.R.J. Vos and N.A.J.M. Sommerdijk and R.J.M. Nolte and J.A. Jansen",

year = "2008",

language = "English",

volume = "361-363",

pages = "605--608",

journal = "Key Engineering Materials",

issn = "1013-9826",

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TY - JOUR

T1 - DNA-coatings: Bioactive properties and effects on osteoblast-like cells

AU - Beucken, van den, J.J.J.P.

AU - Walboomers, X.F.

AU - Leeuwenburgh, S.G.G.

AU - Vos, M.R.J.

AU - Sommerdijk, N.A.J.M.

AU - Nolte, R.J.M.

AU - Jansen, J.A.

PY - 2008

Y1 - 2008

N2 - The aim of this study was to evaluate potential effects of DNA-coatings on calcium phosphate (CaP) nucleation from simulated body fluids (SBF) and subsequently the effects of DNA-coatings and SBF-immersed DNA coatings on the behavior of osteoblast-like cells. DNAcoatings demonstrated to enhance the nucleation and deposition of CaP from SBF compared to titanium controls. The behavior of osteoblast-like cells was affected on SBF-immersed DNAcoatings, showing an increased deposition of the extracellular matrix protein osteocalcin compared to titanium controls. These results indicate bone-bonding capacity of DNA-coatings, which needs to be confirmed using future animal experiments.

AB - The aim of this study was to evaluate potential effects of DNA-coatings on calcium phosphate (CaP) nucleation from simulated body fluids (SBF) and subsequently the effects of DNA-coatings and SBF-immersed DNA coatings on the behavior of osteoblast-like cells. DNAcoatings demonstrated to enhance the nucleation and deposition of CaP from SBF compared to titanium controls. The behavior of osteoblast-like cells was affected on SBF-immersed DNAcoatings, showing an increased deposition of the extracellular matrix protein osteocalcin compared to titanium controls. These results indicate bone-bonding capacity of DNA-coatings, which needs to be confirmed using future animal experiments.

KW - DNA-coating

KW - mineral deposition

KW - calcium phosphate

KW - SBF

KW - cell culture

M3 - Article

VL - 361-363

SP - 605

EP - 608

JO - Key Engineering Materials

JF - Key Engineering Materials

SN - 1013-9826

ER -