Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1660-1667 |
| Number of pages | 8 |
| Journal | Electrophoresis |
| Volume | 25 |
| Issue number | 10-11 |
| DOIs | |
| Publication status | Published - 2004 |
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Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection. / Vrouwe, E.X.; Luttge, R.; Berg, van den, A.
In: Electrophoresis, Vol. 25, No. 10-11, 2004, p. 1660-1667.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection
AU - Vrouwe, E.X.
AU - Luttge, R.
AU - Berg, van den, A.
PY - 2004
Y1 - 2004
N2 - The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
AB - The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
U2 - 10.1002/elps.200405885
DO - 10.1002/elps.200405885
M3 - Article
C2 - 15188255
VL - 25
SP - 1660
EP - 1667
JO - Electrophoresis
JF - Electrophoresis
SN - 0173-0835
IS - 10-11
ER -