Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection

E.X. Vrouwe, R. Luttge, A. Berg, van den

Research output: Contribution to journalArticleAcademicpeer-review

56 Citations (Scopus)

Abstract

The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Original languageEnglish
Pages (from-to)1660-1667
Number of pages8
JournalElectrophoresis
Volume25
Issue number10-11
DOIs
Publication statusPublished - 2004

Fingerprint

Microchip Electrophoresis
Capillary electrophoresis
Capillary Electrophoresis
Lithium
Blood
Electroosmosis
Alkali Metals
Equipment and Supplies
Cells
Cell Separation
Anticoagulants
Fingers
Glass
Limit of Detection
Potassium
Electrophoresis
Erythrocytes
Sodium
Ions
Coatings

Cite this

@article{87faa8436e804d65a48c9457bb5581df,
title = "Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection",
abstract = "The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. {\circledC} 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.",
author = "E.X. Vrouwe and R. Luttge and {Berg, van den}, A.",
year = "2004",
doi = "10.1002/elps.200405885",
language = "English",
volume = "25",
pages = "1660--1667",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "10-11",

}

Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection. / Vrouwe, E.X.; Luttge, R.; Berg, van den, A.

In: Electrophoresis, Vol. 25, No. 10-11, 2004, p. 1660-1667.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Direct measurement of lithium in whole blood using microchip capillary electrophoresis with integrated conductivity detection

AU - Vrouwe, E.X.

AU - Luttge, R.

AU - Berg, van den, A.

PY - 2004

Y1 - 2004

N2 - The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

AB - The direct measurement of lithium in whole blood is described. Using microchip capillary electrophoresis (CE) with defined sample loading and applying the principles of column coupling, alkali metals were determined in a drop of whole blood. Blood collected from a finger stick was mixed with anticoagulant and transferred onto the chip without extraction or removal of components. The electrokinetic transport of red blood cells inside the channels was studied to find sample loading conditions suitable for the analysis of lithium without injecting cells into the separation channel. Both bare glass chips and chips coated with polyacrylamide were used showing the behavior of the cells under different electroosmotic flow conditions. In serum a detection limit for lithium of 0.4 mmol/L was reached. Proteins quickly contaminated untreated chip surfaces but devices with coating gave reproducible electropherograms. In addition, potassium and sodium were also detected in the same separation run. To our knowledge, this is the first device to directly measure ions in whole blood with the use of capillary zone electrophoresis on a microchip. © 2004 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

U2 - 10.1002/elps.200405885

DO - 10.1002/elps.200405885

M3 - Article

C2 - 15188255

VL - 25

SP - 1660

EP - 1667

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 10-11

ER -