Dimerization of papain induced by mercuric chloride and a bifunctional organic mercurial

L.A.A.E. Sluyterman, J. Wijdenes, G. Voorn

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)
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Abstract

The bifunctional mercurial meso-1,4-bis(acetatomercuri)-2,3-diethoxybutane and mercuric chloride are capable of dimerizing papain, by the attachment of the thiol group of two molecules of papain to each molecule of reagent. This is evident from the titration data, gel filtration and sedimentation equilibrium. The conformational change of papain necessary for this reaction is discussed.
Original languageEnglish
Pages (from-to)107-111
Number of pages5
JournalEuropean Journal of Biochemistry
Volume77
Issue number1
DOIs
Publication statusPublished - 1977

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