Differences in fetal bovine serum affect the responsiveness of cells to mechanical loads

M. Rubert, J.R. Vetsch, L. Storz, I. Lehtoviita, R.M. Mueller, S. Hofmann

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Abstract

Nowadays, the end-point of a cell culture in bone tissue engineering
(BTE) is the acquisition of a well mineralized extracellular
matrix. The biological performance of BTE relies on evaluation of
the cell capacity to proliferate and to produce extracellular matrix by
quantification of gene expression and by histology or calcium
quantification assays. Micro-computed tomography (micro-CT) allows
monitoring of BTE mineral constructs in a non-destructive
manner. Although fetal bovine serum (FBS) is commonly used as
supplement in cell cultures, its high composition variability between
different brands and batches leads to differences in the experimental
outcomes. Nevertheless, only few studies have focused on a systematic
investigation of the differences. While we have recently
reported the influence of FBS type on matrix mineralization under
static culture conditions, it is still unknown how FBS affects cells in
dynamic cultures. Different FBS types were used to differentiate
human mesenchymal stem cells down the osteogenic lineage under
dynamic spinner-flask bioreactors. Opposite to static culture conditions,
differences in FBS affected the responsiveness of cells to
differentiate under mechanical loads. Although all FBS types upregulated
the expression of bone-specific genes, differences in the
osteogenic differentiation stage were observed among the different
FBS. Accordingly, micro-CT analysis only showed mineral deposition
for cultures in an advanced differentiation stage.
Thus the selection of the FBS type is crucial for the success in the
acquisition of BTE constructs. The combination of micro-CT with
molecular biology techniqueswill benefit efforts to optimize scaffolds
design and cell culture conditions for scaling-up the BTE constructs.
Original languageEnglish
Pages (from-to)S401-S401
JournalTissue engineering. Part A
Volume21
Issue numberS1
Publication statusPublished - 1 Sep 2015
Event2015 4th TERMIS World Congress, 8-11 September 2015, Boston, Massachusetts, USA - Boston, United States
Duration: 8 Sep 201511 Sep 2015
https://www.termis.org/meetings_worldcongress.php

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