Development of a Novel Approach to Studying Corneodesmosomes and Stratum Corneum Adhesion: Extending Knowledge on the Pathophysiology of Sensitive Skin

Renée J.H. Richters, Natallia E. Uzunbajakava, Nadya Timofeeva, Peter C.M. Van De Kerkhof, Piet E.J. Van Erp

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Background/Aims: Aberrant skin barrier and intercorneocyte adhesion are potential contributors to the pathomechanism of sensitive skin (SS). Here we aimed to develop a novel and easy-to-apply method to analyze corneodesmosomes and to interrogate potential differences between corneocytes of subjects with SS and non-SS (NSS). Methods: Corneocytes of the volar forearm and upper outer quadrant of the left buttock of SS (n = 10) and NSS (n = 8) subjects were extracted as a function of depth using adhesive tape and stained with anti-desmoglein 1 (DSG1) antibody. The total area of corneocytes and the number and average size of cells per tape was estimated using image processing. Results: The total area of extracted corneocytes and the quantity of DSG1 decreased with depth. The level of decrease, total area of corneocytes, and average area of individual cells differed between anatomical locations. In SS, a larger total area of extracted corneocytes and a larger average cell size per tape was found at all inspected depths. Conclusion: The developed novel and easy-to-apply approach allows investigation of corneodesmosome components. We confirm a role of altered corneocytes in the pathomechanism of SS. The disclosed protocol can further be optimized in studies of skin conditions with strongly affected corneodesmosomes.

Original languageEnglish
Pages (from-to)81-93
Number of pages13
JournalSkin Pharmacology and Physiology
Volume32
Issue number2
DOIs
Publication statusPublished - 1 Mar 2019
Externally publishedYes

Keywords

  • Corneodesmosomes adhesion
  • Image analysis
  • Sensitive skin
  • Stratum corneum adhesion
  • Tape stripping

Fingerprint Dive into the research topics of 'Development of a Novel Approach to Studying Corneodesmosomes and Stratum Corneum Adhesion: Extending Knowledge on the Pathophysiology of Sensitive Skin'. Together they form a unique fingerprint.

Cite this