Development of a lab-on-a-disk platform with digital imaging for identification and counting of parasite eggs in human and animal stool

Sertan Sukas, Bieke van Dorst, Agata Kryj, Ole Lagatie, Wim De Malsche (Corresponding author), Lieven J. Stuyver

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

We present a lab-on-a-disk technology for fast identification and quantification of parasite eggs in stool. We introduce a separation and packing method of eggs contained in 1 g of stool, allowing for removal of commonly present solid particles, fat droplets and air bubbles. The separation is based on a combined gravitational and centrifugal flotation, with the eggs guided to a packed monolayer, enabling quantitation and identification of subtypes of the eggs present in a single field of view (FOV). The prototype was tested with stool samples from pigs and humans infected with intestinal parasites (soil-transmitted helminths eggs). The quality of the images created by this platform was appropriate for identification and quantification of egg types present in the sample.

Original languageEnglish
Article number852
Number of pages15
JournalMicromachines
Volume10
Issue number12
DOIs
Publication statusPublished - 1 Dec 2019
Externally publishedYes

Bibliographical note

Funding Information:
Funding: This research was funded by Jansen Global Public Health (JGPH).

Publisher Copyright:
© 2019 by the authors.

Keywords

  • Diagnostic microfluidic device
  • Parasite egg identification and quantification
  • Particle separation

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