Development of a lab-on-a-disk platform with digital imaging for identification and counting of parasite eggs in human and animal stool

Sertan Sukas; Bieke van Dorst; Agata Kryj; Ole Lagatie; Wim De Malsche; Lieven J. Stuyver

doi:10.3390/mi10120852

Development of a lab-on-a-disk platform with digital imaging for identification and counting of parasite eggs in human and animal stool

Sertan Sukas, Bieke van Dorst, Agata Kryj, Ole Lagatie, Wim De Malsche (Corresponding author), Lieven J. Stuyver

Research output: Contribution to journal › Article › Academic › peer-review

13 Citations (Scopus)

Abstract

We present a lab-on-a-disk technology for fast identification and quantification of parasite eggs in stool. We introduce a separation and packing method of eggs contained in 1 g of stool, allowing for removal of commonly present solid particles, fat droplets and air bubbles. The separation is based on a combined gravitational and centrifugal flotation, with the eggs guided to a packed monolayer, enabling quantitation and identification of subtypes of the eggs present in a single field of view (FOV). The prototype was tested with stool samples from pigs and humans infected with intestinal parasites (soil-transmitted helminths eggs). The quality of the images created by this platform was appropriate for identification and quantification of egg types present in the sample.

Original language	English
Article number	852
Number of pages	15
Journal	Micromachines
Volume	10
Issue number	12
DOIs	https://doi.org/10.3390/mi10120852
Publication status	Published - 1 Dec 2019
Externally published	Yes

Bibliographical note

Funding Information:
Funding: This research was funded by Jansen Global Public Health (JGPH).

Publisher Copyright:
© 2019 by the authors.

Keywords

Diagnostic microfluidic device
Parasite egg identification and quantification
Particle separation

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10.3390/mi10120852

Cite this

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title = "Development of a lab-on-a-disk platform with digital imaging for identification and counting of parasite eggs in human and animal stool",

abstract = "We present a lab-on-a-disk technology for fast identification and quantification of parasite eggs in stool. We introduce a separation and packing method of eggs contained in 1 g of stool, allowing for removal of commonly present solid particles, fat droplets and air bubbles. The separation is based on a combined gravitational and centrifugal flotation, with the eggs guided to a packed monolayer, enabling quantitation and identification of subtypes of the eggs present in a single field of view (FOV). The prototype was tested with stool samples from pigs and humans infected with intestinal parasites (soil-transmitted helminths eggs). The quality of the images created by this platform was appropriate for identification and quantification of egg types present in the sample.",

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AU - Lagatie, Ole

AU - De Malsche, Wim

AU - Stuyver, Lieven J.

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Development of a lab-on-a-disk platform with digital imaging for identification and counting of parasite eggs in human and animal stool

Abstract

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Keywords

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