Determination of uric acid in serum using isotachophoresis

T.M.M. Verheggen, F.E.P. Mikkers, F.M. Everaerts, F. Oerlemans, C.H.M.M. Bruijn, de

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)
212 Downloads (Pure)

Abstract

An operational system is described for the isotachophoretic determination of uric acid in serum, making use of column coupling. The method has been compared with a standard enzymatic procedure. With the present technique small amounts of serum (ca. 3 μl) can be applied without any pretreatment. Urate recovery was 99.0–100.5%. Under the non-physiological measuring conditions used, 12–28% of control serum uric acid was bound to macromolecules of molecular weight exceeding 25,000. The day-to-day variations of the isotachophoretic procedure were smaller than those of the enzymatic method, whereas standard deviations were comparable. The isotachophoretic procedure is less influenced by certain metabolites.
Original languageEnglish
Pages (from-to)317-324
Number of pages8
JournalJournal of Chromatography, B: Biomedical Sciences and Applications
Volume182
Issue number3
DOIs
Publication statusPublished - 1980

Fingerprint Dive into the research topics of 'Determination of uric acid in serum using isotachophoresis'. Together they form a unique fingerprint.

  • Cite this

    Verheggen, T. M. M., Mikkers, F. E. P., Everaerts, F. M., Oerlemans, F., & Bruijn, de, C. H. M. M. (1980). Determination of uric acid in serum using isotachophoresis. Journal of Chromatography, B: Biomedical Sciences and Applications, 182(3), 317-324. https://doi.org/10.1016/S0378-4347(00)81480-2