An operational system is described for the isotachophoretic determination of uric acid in serum, making use of column coupling. The method has been compared with a standard enzymatic procedure. With the present technique small amounts of serum (ca. 3 μl) can be applied without any pretreatment. Urate recovery was 99.0–100.5%. Under the non-physiological measuring conditions used, 12–28% of control serum uric acid was bound to macromolecules of molecular weight exceeding 25,000. The day-to-day variations of the isotachophoretic procedure were smaller than those of the enzymatic method, whereas standard deviations were comparable. The isotachophoretic procedure is less influenced by certain metabolites.
|Number of pages||8|
|Journal||Journal of Chromatography, B: Biomedical Sciences and Applications|
|Publication status||Published - 1980|
Verheggen, T. M. M., Mikkers, F. E. P., Everaerts, F. M., Oerlemans, F., & Bruijn, de, C. H. M. M. (1980). Determination of uric acid in serum using isotachophoresis. Journal of Chromatography, B: Biomedical Sciences and Applications, 182(3), 317-324. https://doi.org/10.1016/S0378-4347(00)81480-2